Folia Microbiologica

, Volume 30, Issue 3, pp 247–257 | Cite as

Antibacterial immunity of lower airways: local or localized?

  • J. Franěk
  • J. Libich
  • J. Malina


Clearance of bacteria in the bronchoalveolar lavage, the level and functional activity of IgA and changes in the cellular composition of BAL were examined in mice after supralaryngeal immunization and subsequent challenge withKlebsiella pneumoniae. More than 60 % of the bacterial inoculum was removed by nonspecific mechanisms within 90 min after inoculation; within the time interval 1.5 – 3.5 h, clearance was significantly accelerated in locally immunized mice. The enhancement of clearance effectiveness is specific and increases proportionally with the length of immunization (1 < 2 < 4 weeks); it is of short duration and towards the end of the 3rd week after immunization, in 73 % of immunized animals, the clearance values did not differ from values found in controls. The local immunization did not influence the total level of IgA in BAL, the formation of specific IgA antibody was minimal,in vivo binding of IgA to klebsiella could not be demonstrated. In immunized mice, a significant increase in the numbers of PMN and lymphocytes, as well as an increased activity of phagocytic cell (PMN, MP) was found in BAL. The time interval of 1.5–3.5 h after challenge bounds the space for mechanisms, activated by local immunization in lower airways. The actual participation of individual factors in the accelerated elimination of bacteria from the lumen of airways, remains unclear so far.


Lower Airway Immunize Mouse Mucosal Immunity System Local Immune Response Clearance Effectiveness 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



bronchoalveolar lavage fluid


bronchus-associated lymphoid tissue


immunofluorescent, slgA secretory IgA


lower airways


mucosal immunity system




polymorphonuclear leukocytes


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  1. Bienenstoc K J., Befus A.D.: Some thoughts on the biologic role of immunoglobulin A.Gastroenterology 84, 178 (1983).Google Scholar
  2. Calvanico N.J., Ambegaonkar S., Geoghegan W.D., Hanly W.C.: Analysis of rabbit lung lavage immunoglobulins during the course of pulmonary inflamation induced with aerosolized antigen.Clin. Exp. Immunol. 56, 302 (1984).PubMedGoogle Scholar
  3. Challacombe S.J.: Salivary antibodies and systemic tolerance in mice after oral immunization vith bacterial antigens, inThe Secretory Immune System (J.R. McGhee, J. Mestecky, eds.).Ann. N.Y. Acad. Sci. 409, 177 (1983).Google Scholar
  4. Cooper J. McA., Finlay-Jones J.J., Hill N.L., Rowley D.: Local immunity toKlebsiella pneumoniae in the lungs of mice.J. Infect. Dis. 147, 312 (1983).PubMedGoogle Scholar
  5. Cbyz S.J. Jr.,Furer E., Germanier R.: ExperimentalKlebsiella pneumoniae burn wound sepsis: role of capsular polysaccharide.Infect. Immun. 43, 440 (1984).Google Scholar
  6. Fick R.B., Reynolds H.Y.: Pseudomonas respiratory infection in cystic fibrosis: a possible defect in opsonie IgG antibody?Bull. Europ. Physiopath. Resp. 19, 151 (1983).Google Scholar
  7. Franěk J., Libich J., Kubín V.: Mechanisms of antibacterial immunity of mucous membranes.Folia Microbiol. 29, 375 (1984).CrossRefGoogle Scholar
  8. Ganguly R., Waidmas R.H.: Immunology of respiratory viruses, p. 165 inImmunology of Human Infection, Part II (A. J. Nahmias, R. J. O’Reilly, eds.). Plenum Medical Book Co., New York-London 1981.Google Scholar
  9. Goldstein E., Bartlema H.C.: Role of the alveolar macrophage in pulmonary bacterial defense.Bull. Europ. Physiopath. Resp. 13, 57 (1977).Google Scholar
  10. Gregson R.L., Davey M.J., Frentice D.E.: The response of rat bronchus-associated lymphoid tissue to local antigenic challenge.Brit. J. Exp. Path. 60, 471 (1979).PubMedGoogle Scholar
  11. Hudson A.R., Kilburn K.H., Halprin G.M., McKenzie W.N.: Granulocyte recruitment to airways exposed to endotoxin aerosols.Ann. Rev. Respir. Dis. 115, 89 (1977).Google Scholar
  12. Kaltreider H.B.: Expression of immune mechanisms in the lung.Ann. Rev. Respir. Dis. 113, 347 (1976).Google Scholar
  13. La Force F.M.: Effect of aerosol immunization with RE 595Salmonella minnesota on lung bactericidal activity againstSerratia marcescens, Enterobacter cloacae, andPseudomonas aeruginosa.Am. Rev. Respir. Dis. 116, 241 (1977).Google Scholar
  14. La Force F.M., Boose D.S., Mills D.M.: Heightened lung bacterial activity in mice after aerosol immunization with RE 595Salmonella minnesota: importance of cellular rather than humoral factors.J. Infect. Dis. 142, 421 (1980).Google Scholar
  15. Lamm M.E., Rocex E., McWilliams M., Phillips-Quagliata J.M.: Lymphocyte traffic in the secretory immune system of the gut, p. 36 inThe Mucosal Immune System in Health and Disease (P. L. Ogra, J. Bienenstock, eds.). Ross Laboratories, Columbus (Ohio) 1981.Google Scholar
  16. Libich J., Franěk J., Hásková V.: Experimental model ofKlebsiella infection in mice for studies of mechanisms of local immunity of the respiratory tract.Folia Microbiol. 28, 424 (1983a).CrossRefGoogle Scholar
  17. Libich J., Franěk J., Tučková L.: Modification of Oudin’s method of single immunodiffusion in agar gel for detection of IgA in bronchoalveolar lavage of white mice.Folia Microbiol. 28, 430 (1983b).CrossRefGoogle Scholar
  18. Pennington J.E., Pier G.B.: Efficacy of cell wallPseudomonas aeruginosa vaccines for protection against experimental pneumonia.Rev. Infect. Dis. 5, Suppl. 5, S 852 (1983).Google Scholar
  19. Pierce N.F., Cray W.C., Sircar B.K.: Induction of a mueosal antitoxin response and its role in immunity to experimental canine cholera.Infect. Immun. 21, 185 (1978).PubMedGoogle Scholar
  20. Pierce A.K., Reynolds R.C., Harris G.D.: Leukocytic response to inhaled bacteria.Am. Rev. Respir. Dis. 116, 679 (1977).PubMedGoogle Scholar
  21. Rehm S.R., Gross G.N., Pierce A.K.: Early bacterial clearance from murine lungs.J. Clin. Invest. 66, 194 (1980).PubMedCrossRefGoogle Scholar
  22. Reynolds H. Y., KazmieroWski J. A., NeWball H.H.: Specificity of opsonie antibodies to enhance phagocytosis ofPseudomonas aeruginosa by human alveolar macrophages.J. Clin. Invest. 56, 376 (1975).PubMedCrossRefGoogle Scholar
  23. Robbins J.H., Schneerson R.: Planning for a second (23 valent) generation of pneumococcal vaccine.Bull. Europ. Physiopath. Resp. 19, 215 (1983).Google Scholar
  24. Sparling P.F.: Bacterial virulence and pathogenesis: an overview.Rev. Infect. Dis. 5, Suppl. 4, S 637 (1983).Google Scholar
  25. Větvička V., Fornůsek L., Kopeček J., PřikrylovÁ D.: Phagocytosis of 2-hydroxyethylmethacrylate copolymer particles by different types of macrophages.Folia Biol. (Prague) 29, 424 (1983).Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1985

Authors and Affiliations

  • J. Franěk
    • 1
  • J. Libich
    • 1
  • J. Malina
    • 1
  1. 1.Department of Immunology, Institute of Experimental MedicineCzechoslovak Academy of SciencesPrague 2

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