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Biologia Plantarum

, Volume 18, Issue 5, pp 397–400 | Cite as

The effects of methyl methanesulphonate on mitosis and chromosomes of excised barley embryos

  • T. Gichner
  • M. Ondřej
  • Lim En Se
  • J. Velemínský
Brief Communication

Abstract

Excised barley embryos were treated with methyl methanesulphonate and cultivatedin vitro in water or in a nutrient medium. The alkylating compound induced a high frequency of chromosome aberrations observed at anaphase and metaphase and depressed the mitotic division in the root-tips of excised embryos.

Keywords

Root Length Nutrient Medium Mitotic Index Chromosome Aberration Mitotic Division 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Lescure, A. M.: Mutagenèse et sélection de cellules d’Acer pseudoplatanus L. cultivéesin vitro. — Physiol. vég.7: 237–250, 1969.Google Scholar
  2. Miflin, B. J.: A technique for the sterile culture of germinating barley embryos. — J. exp. Bot.20: 805–809, 1969.CrossRefGoogle Scholar
  3. Van’t Hof, J.: Postirradiation hypoxia and the reoovery of G1 and G2 cells and DNA synthesis in cultured root meristems. — Radiat. Res.41: 538–551, 1970.CrossRefGoogle Scholar
  4. Webster, P. L., Van’t Hof, J.: Recovery of G2 cells in pea root meristems; survival and mitotie delay following irradiation. — Radiat. Bot.10: 145–154, 1970.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany 1976

Authors and Affiliations

  • T. Gichner
    • 1
  • M. Ondřej
    • 1
  • Lim En Se
    • 1
    • 2
  • J. Velemínský
    • 1
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of ScienoesPraha 6Czechoslovakia
  2. 2.Institute of Experimental BiologyAoademy of SoiencesPyongyangDemocratic People’s Republic of Korea

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