Abstract
Pig muscle aldolase was covalently attached to a polyacrylamide support possessing carboxylic functional groups activated by various water-soluble disubstituted carbodiimides. The stability and the degree of microheterogeneity of the immobilized aldolases were shown to depend on the structure of the carbodiimide used as coupling agent.
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Ábrahám, M., Pénzes, Z. & Szajáni, B. Effects of carbodiimides as coupling agents on the stability of immobilized aldolases. Appl Biochem Biotechnol 33, 25–32 (1992). https://doi.org/10.1007/BF02922181
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DOI: https://doi.org/10.1007/BF02922181