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Effects of carbodiimides as coupling agents on the stability of immobilized aldolases

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Abstract

Pig muscle aldolase was covalently attached to a polyacrylamide support possessing carboxylic functional groups activated by various water-soluble disubstituted carbodiimides. The stability and the degree of microheterogeneity of the immobilized aldolases were shown to depend on the structure of the carbodiimide used as coupling agent.

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Authors and Affiliations

  1. Department of Biochemistry, Attila József University, PO Box 533, H-6721, Szeged, Hungary

    Magdolna Ábrahám

  2. Institute of Biophysics, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary

    Zsolt Pénzes

  3. Reanal Factory of Laboratory Chemicals, PO Box 54, Budapest 70, Hungary

    Béla Szajáni

Authors
  1. Magdolna Ábrahám
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  2. Zsolt Pénzes
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  3. Béla Szajáni
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Ábrahám, M., Pénzes, Z. & Szajáni, B. Effects of carbodiimides as coupling agents on the stability of immobilized aldolases. Appl Biochem Biotechnol 33, 25–32 (1992). https://doi.org/10.1007/BF02922181

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  • DOI: https://doi.org/10.1007/BF02922181

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