Abstract
Yeast invertase, a glycoprotein, was covalently coupled toOcimum basilicum seeds either through its protein or carbohydrate moiety. Of the various methods investigated, binding of the enzyme through its carbohydrate moiety resulted in the retention of considerably higher amounts of enzyme activity. Immobilized invertase showed a shift in the pH optimum toward the alkaline side without appreciable change in temperature optimum. However, the immobilized preparation was more thermostable than the free enzyme. Invertase bound to the seeds could be used repeatedly for the hydrolysis of sucrose syrups in a batch process without appreciable loss in activity. The seeds could serve as an inexpensive, ready-to-use, natural pellicular polysaccharide support for immobilizing enzymes.
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Melo, J.S., D’sogza, S.F. Immobilization of invertase through its carbohydrate moiety onOcimum basilicum seed. Appl Biochem Biotechnol 32, 159–170 (1992). https://doi.org/10.1007/BF02922156
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DOI: https://doi.org/10.1007/BF02922156