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Activation and detection of (Pro)mutagenic chemicals using recombinant strains ofStreptomyces griseus

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Abstract

Two recombinant strains ofStreptomyces griseus have been developed to report on the activation of promutagenic° chemicals. This activation is monitored by reversion of the bacterial test strains to a kana-mycin-resistant phenotype. Strain H69 detects point mutations and was reverted at an increased frequency by acetonitrile, 2-aminoanthracene, 1,2-benzanthracene, benzidine, benzo(a)pyrene, 9,10-dimethyl-1,2-benzanthracene, and glycine. The second strain, FS2, detects frame shift mutations and was reverted at an increased frequency by 1,2-benzanthracene, benzidine, and glycine. Compounds such as butylated hydroxytoluene, catechol, chlorobenzene, hydroquinone, potassium chloride, phenol,cis-stilbene,trans-stilbene, and toluene did not elicit positive responses in either strain. In addition, these strains are capable of detecting direct-acting mutagens such asN-methyl-N’-nitrosoguanidine and ICR-191, providing further evidence of their promise for detecting a wider range of mutagens. To our knowledge, this is the first report of bacterial strains capable of activating promutagenic compounds and detecting their mutagenic metabolites without the benefit of an exogenous activation system such as the rodent liver homogenate (S9).

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Author notes

  1. Steven E. Buchholz

    Present address: Biopress Dev. Dept, Hoffman LaRoche Inc., 07110, Nutley, NJ

Authors and Affiliations

  1. Central Research and Development, Experimental Station and E. I. du Pont de Nemours & Co, PO Box 80228, 19880-0228, Wilmington, DE

    Steven E. Buchholz, Charles A. Omer, Paul V. Viitanen & F. Slma Sariaslani

  2. Central Research and Development, Haskell Laboratory for Toxicology and Industrial Medicine, PO Box 80228, 19880-0228, Wilmington, DE

    Ralph G. Stahl

Authors
  1. Steven E. Buchholz
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  2. Charles A. Omer
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  3. Paul V. Viitanen
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  4. F. Slma Sariaslani
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  5. Ralph G. Stahl
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Buchholz, S.E., Omer, C.A., Viitanen, P.V. et al. Activation and detection of (Pro)mutagenic chemicals using recombinant strains ofStreptomyces griseus . Appl Biochem Biotechnol 32, 149–158 (1992). https://doi.org/10.1007/BF02922155

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  • DOI: https://doi.org/10.1007/BF02922155

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