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Partial purification and some properties of a hemolymph lectin from panstrongylus megistus (Hemiptera, Reduviidae)

  • Yara De M. Gomes
  • AndrÉ F. Furtado
  • Luana B. B. Coelho
Article

Abstract

Hemagglutinating activity was studied in homogenates of three embryonic stages, and in the hemolymph of most instar larvae and in adult insects ofPanstrongylus megistus, an important Chagas’ disease vector in Brazil. A hemolymph lectin from the 5th instar larvae ofP. megistus was purified through a biospecific adsorption by using formaldehyde-treated erythrocytes. The lectin fraction was desorbed with 0.2M D-galactose in 0.15M NaCl. The lectin fraction activity was inhibited by L-rhamnose, D-lactose, raffinose, D-galactose, and Dfucose. The electrophoretic pattern to native and acidic proteins resolved lectin fraction in two main bands with lectin activity. These bands were considered as multiple molecular forms or isoforms ofP. megistus lectin. Under denaturating conditions, isoform 1 showed one band with apparent mol wt (MW) of 64 kDa while isoform 2 was resolved in two bands with MW of 64 and 33 kDa.

Keywords

Apply Biochemistry Raffinose Instar Larva Embryonic Stage Hemocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • Yara De M. Gomes
    • 1
  • AndrÉ F. Furtado
    • 1
  • Luana B. B. Coelho
    • 2
  1. 1.Laboratório de ImunologiaCentro de Pesquisas Aggeu MagalhāesRecifeBrazil
  2. 2.Departamento de BioquímicaCentro de Ciencias BiológicasBrazil

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