Factors affecting stability ofSclerotium rolfsii UV-8 mutant cellulase complex under saccharification conditions

Abstract

Enzyme stability studies in case ofSclerotium rolfsii UV-8 mutant have been investigated under the conditions used for saccharification of cellulose (50°C, pH 4.5, 48 h). Avicelase (measure of exoenzymes) and xylanase were found to be less stable than CMCase (endoglucanase) and β-glucosidase. Merthiolate (and other Hg compounds) added as a biocide, inactivated avicelase and xylanase about 60-70%. Of the antibiotics tested, tetracycline, chloramphenicol, and streptomycin sulfate were found suitable as an additive in cellulose hydrolysis system. The optimum hydrolysis of alkali-treated (AT)-rice straw, ATbagasse, Solka Floc SW40, and Avicel P.H.101 was observed under shaking conditions at pH 4.5, 50°C in CO2 atmosphere. It is suggested, all the studied parameters could be used for the evaluation of mutant strains.

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Correspondence to Mukund V. Deshpande.

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Deshpande, M.V. Factors affecting stability ofSclerotium rolfsii UV-8 mutant cellulase complex under saccharification conditions. Appl Biochem Biotechnol 31, 75–82 (1991). https://doi.org/10.1007/BF02922127

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Keywords

  • Cellulase
  • Straw
  • Apply Biochemistry
  • Bagasse
  • Rice Straw