Factors affecting stability ofSclerotium rolfsii UV-8 mutant cellulase complex under saccharification conditions
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Enzyme stability studies in case ofSclerotium rolfsii UV-8 mutant have been investigated under the conditions used for saccharification of cellulose (50°C, pH 4.5, 48 h). Avicelase (measure of exoenzymes) and xylanase were found to be less stable than CMCase (endoglucanase) and β-glucosidase. Merthiolate (and other Hg compounds) added as a biocide, inactivated avicelase and xylanase about 60-70%. Of the antibiotics tested, tetracycline, chloramphenicol, and streptomycin sulfate were found suitable as an additive in cellulose hydrolysis system. The optimum hydrolysis of alkali-treated (AT)-rice straw, ATbagasse, Solka Floc SW40, and Avicel P.H.101 was observed under shaking conditions at pH 4.5, 50°C in CO2 atmosphere. It is suggested, all the studied parameters could be used for the evaluation of mutant strains.
KeywordsCellulase Straw Apply Biochemistry Bagasse Rice Straw
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- 1.Sadana, J. C., Shewale, J. G., and Deshpande, M. V. (1980), inProc. II Symp. on Bioconversion and Biochemical Engineering, vol. 1, Ghosh, T. K., ed., I.I.T., New Delhi, India, pp. 455–484.Google Scholar
- 3.Parisi, F. (1989), inAdvances in Biochemical Engineering/Biotechology, vol. 38, Fiechter, A. ed., Springer-Verlag, New York, pp. 53–87.Google Scholar
- 5.Lynd, L. R. (1989), inAdvances in Biochemical Engineering/Biotechnology, vol. 38, Fiechter, A. ed., Springer-Verlag, New York, pp. 1–52.Google Scholar
- 7.Sadana, J. C., Shewale, J. G., and Deshpande, M. V. (1979),Appl. Environ. Microbiol. 38, 730.Google Scholar
- 9.Wood, T. M. and Bhat, K. M. (1988), inMethods in Enzymology, vol. 160, Wood, W. A. and Kellogg, S. T., eds.,Academic, New York, pp. 87–112.Google Scholar
- 11.Report number 1760302 Ethanol AL. Swedish Board for Energy Conservation, 1983.Google Scholar
- 14.Sinitsyn, A. P., Mitkevich, O. V., and Klesov, A. A. (1986),Prikl. Biochim. Mikrobiol. 22, 759.Google Scholar