Applied Biochemistry and Biotechnology

, Volume 31, Issue 1, pp 75–82 | Cite as

Factors affecting stability ofSclerotium rolfsii UV-8 mutant cellulase complex under saccharification conditions

  • Mukund V. Deshpande


Enzyme stability studies in case ofSclerotium rolfsii UV-8 mutant have been investigated under the conditions used for saccharification of cellulose (50°C, pH 4.5, 48 h). Avicelase (measure of exoenzymes) and xylanase were found to be less stable than CMCase (endoglucanase) and β-glucosidase. Merthiolate (and other Hg compounds) added as a biocide, inactivated avicelase and xylanase about 60-70%. Of the antibiotics tested, tetracycline, chloramphenicol, and streptomycin sulfate were found suitable as an additive in cellulose hydrolysis system. The optimum hydrolysis of alkali-treated (AT)-rice straw, ATbagasse, Solka Floc SW40, and Avicel P.H.101 was observed under shaking conditions at pH 4.5, 50°C in CO2 atmosphere. It is suggested, all the studied parameters could be used for the evaluation of mutant strains.


Cellulase Straw Apply Biochemistry Bagasse Rice Straw 
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Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • Mukund V. Deshpande
    • 1
  1. 1.Biochemical Sciences DivisionNational Chemical LaboratoryPuneIndia

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