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Applied Biochemistry and Biotechnology

, Volume 31, Issue 1, pp 53–58 | Cite as

Immobilization of protein on ferromagnetic Dacron

  • A. M. A. Carneiro LeÃo
  • E. A. Oliveira
  • L. B. Carvalho
Article

Abstract

Ferromagnetic Dacron (polyethyleneterephthalate) is proposed as a matrix to immobilize proteins covalently. Dacron in powder was magnetized by reacting ferrous (Fe+2) and ferric (Fe+3) ions with its hydrazide form at pH 8.3. Ferromagnetic hydrazide Dacron was converted to ferromagnetic azide Dacron and amyloglucosidase (E.C. 3.2.1.3) was covalently bound through the latter group. The catalytic property of the enzyme was preserved (8% of the specific activity estimated for the soluble enzyme) and all the magnetic amyloglucosidase Dacron derivative was recovered by using a magnetic field. No activity was detected in the supernatant.

Keywords

Apply Biochemistry Hydrazide Hydrazine Hydrate Soluble Starch Polyethyleneterephthalate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • A. M. A. Carneiro LeÃo
    • 1
  • E. A. Oliveira
    • 1
  • L. B. Carvalho
    • 1
  1. 1.Laboratório de Enzimologia do Departamento de Bioquímica & Laboratório de ImunopatologiaKeizo Asami da Universidade Federal de PernambucoRecifeBrazil

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