Abstract
Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob. The immobilization is carried out by tosylation of primary alcohols of cellulose of corn cob. The immobilized benzonase is more stable vs pH changes than native benzonase, but the same optimum values of [Mg(II)] and temperature are obtained. The DNase activity is greater than the RNase activity. The percentage of DNA is reduced to 3-6% and that of RNA to 50%. The protein loss is negligible (1%). The enzymatic activity per weight unit of enzyme is greater in the case of benzonase that in reported data for other nucleases insolubilized on corn cob by the same procedure.
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Bressani, R. and Elias L. C. (1968),Adv. Food Res. 16, 1–7.
Kihlberg, R. (1972),Ann. Rev. Microbiol.26,427–466.
Edozein, J. C, Udo, U. U., Young, V. R., and Scrimshaw, N. S. (1970),Nature (London) 228, 180.
Newell, K. A., Robbins, E. A., and Seeley, R. D. (1975), U.S. Patent 3,867,255.
Otero, M. A., Gonzalez, A. C., Bueno, G. E., and Garcia-Revilla, J. L. (1982),Biotechnol. Lett. 4, 149–152.
Schlenk, I. and Dainko, J. L. (1965),J. Bacteriol. 89, 13–18.
Martinez, M. C., Sanchez-Montero, J. M., Sinisterra, J. V., and Ballesteros, A. (1990),Biotechnol. Appl. Biochem. 12, 643–652.
Technical information: “Benzonase, the first industrial endonuclease” (1989), Alfred Benzon Inc., Merck PA.
Sanchez-Montero, J. M., Sinisterra, J. V., and Ballesteros, A. (1989),Appl. Biochem. Biotechnol. 22, 205–214.
Sanchez-Montero, J. M., Sinisterra, J. V., and Ballesteros, A. (1988), Spanish Patent 8, 803,769.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, J. (1951),J. Biol. Chem. 193, 265–275.
Peterson, G. L. (1979),Anal. Biochem. 100, 201–220.
Schmidt, G. and Thannhauser, S. J. (1945),J. Biol. Chem. 161, 83–89.
Steward, P. R. (1975), inMethods in Cell Biology vol. II, Yeast Cells (Prescott, D., ed.), Academic, New York.
Nielsen, L. B. and Hansen, O. C. (1990), Summary book of 5th European Congress on Biotechnology, p. 247, Copenhagen (Denmark).
Miller, S. A. (1968), inSingle-Cell Protein (Mateles, R. I. and Tannenbaum, S. R., eds.), MIT Press, Cambridge, MA.
Moreno, J. M. (1991), Ph.D. thesis, Universidad Complutense de Madrid, Spain.
Dunnill, P. (1975), inProtein Isolation Single-Cell Protein II (Tannenbaum, S. R. and Wang, D. I. C., eds.), MIT Press, Cambridge, MA.
Alvarez, R. and Enriquez, A. (1988),Appl. Microbiol. Biotechnol.29, 208–210.
Ibid (1984),Rev. Cienc. Biol. (Havana)15, 59–66.
(a) Hendenskog, H. and Mogren, H. (1970),Biotechnol. Bioeng. 2, 447. (b) Ibid (1973), 15, 129.
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Moreno, J.M., Sanchez-Dmontero, J.M., Ballesteros, A. et al. Hydrolysis of nucleic acids in single-cell protein concentrates using immobilized benzonase. Appl Biochem Biotechnol 31, 43–51 (1991). https://doi.org/10.1007/BF02922124
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DOI: https://doi.org/10.1007/BF02922124