Immunological and molecular genetic analysis of the cellulase component fromPenicillium funiculosum
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Following immunization of rabbits, the antiserum was initially analyzed for antiendoglucanase activity using dot-blot ELISA methods. When compared with the preimmune serum, the antiserum showed strong response even at 25 ng concentration. The specificity of the polyclonal antibodies, raised against the partially purified endoglucanase component ofPenicillium funiculosum, was determined by Oüchterlony double diffusion. Rocket electrophoresis confirmed the presence of only two types of antigens in the serum. The two rockets obtained were attributable to endo I and the merging of immunologically identical (but mobilitywise different) endo II and endo III. This antibody preparation was used as a probe. The deduced M1 of the cloned E.coli endo I was found to be 58K d by Western blotting.
KeywordsCellulase Apply Biochemistry Cellulase Gene Antibody Preparation Preimmune Serum
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