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Polyelectrolyte complex capsules as a material for enzyme immobilization

catalytic properties of Encapsulated Lactate Dehydrogenase

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Abstract

The polyelectrolyte complex (PEC) membrane formed by cellulose sulfate and poly(dimethyldiallylammonium chloride) was used to encapsulate lactate dehydrogenase. The exclusion limit of the membrane was found to be low enough to secure irreversible entrapping of the enzyme. The obtained capsules were checked for their functionality in a stirred-batch reactor by following the kinetics of NADH oxidation. The data were fitted with an isotropic kinetic model including competitive product-inhibition phenomenon. The results of mathematical modeling demonstrated that the anisotropic system, like PEC capsules, could be satisfactorily described by the isotropic model.

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Authors and Affiliations

  1. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 842 38, Bratislava, Czech and Slovak Federal Republic (CSFR)

    VladimÍr Štefuca, Peter Gemeiner & Ľubica KurillovÁ

  2. Institute of Polymer Chemistry “Erich Correns”, 1530, Teltow-Seehof, Federal Republic of Germany

    Horst Dautzenberg

  3. Department of Chemical and Biochemical Engineering, Slovak Technical University, 812 37, Bratislava, CSFR

    VladimÍr Štefuca, Milan PolakovLČ & VladimÍr BÁleŠ

Authors
  1. VladimÍr Štefuca
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  2. Peter Gemeiner
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  3. Ľubica KurillovÁ
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  4. Horst Dautzenberg
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  5. Milan PolakovLČ
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  6. VladimÍr BÁleŠ
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Štefuca, V., Gemeiner, P., KurillovÁ, Ľ. et al. Polyelectrolyte complex capsules as a material for enzyme immobilization. Appl Biochem Biotechnol 30, 313–324 (1991). https://doi.org/10.1007/BF02922035

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  • DOI: https://doi.org/10.1007/BF02922035

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