Abstract
Adsorption and expression of penicillin G acylase was studied on macroporous methacrylate polymer beads of differing pore volume, generated with kerosene. The absorption and expression of the penicillin G acylase was dependent on pore volume. Maximum expression of 57% of adsorbed enzyme was obtained on beads synthesized with 40 mL of kerosene, indicating minimum pore-diffusion limitations.
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† NCL Communication Number 4976.
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Kotha, A., Selvaraj, L., Rajan, C.R. et al. Adsorption and expression of penicillin G acylase immobilized onto methacrylate polymers generated with varying pore generating solvent volume. Appl Biochem Biotechnol 30, 297–302 (1991). https://doi.org/10.1007/BF02922033
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DOI: https://doi.org/10.1007/BF02922033