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Production of exocellular polysaccharide by azotobacter chroococcwn

  • M. G. De La Vega
  • F. J. Cejudo
  • A. Paneque
Article

Abstract

Environmental conditions affect the production of extracellular polysaccharide byAzotobacter chroococcum ATCC 4412. Production of exocellular polymer from a variety of carbon sources depended on the air flow rate. A high sucrose concentration in medium (8%) markedly favored exopolysaccharide production, which reached 14 g/L in about 72 h. In cell suspensions incubated in the presence of 8% sucrose in a nitrogen-free medium, biopolymer final concentration of 9 g/L corresponds to 68 g/g biomass. Maximum efficiency of sucrose conversion into exopolysaccharide peaked at 70% for initial disaccharide concentration of 6%. High performance liquid chromatography and gas liquid chromatography of acid hydrolysates of the exopolymer revealed the presence of mannuronosyl, guluronosyl, and acetyl residues, but not neutral sugars. The infrared spectrum corroborated the presence of carboxylate anions and O-acetyl groups in the exopolymer. Though the presence of more than one kind of polysaccharide cannot be ruled out, these data suggest that, under the experimental conditions used in this work, only a type of alginate-like exopolysaccharide is produced byA. chroococcum ATCC 4412.

Index Entries

Alginate carbon sources biotransformation molasses Azotobacter chroococcum 

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Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • M. G. De La Vega
    • 1
  • F. J. Cejudo
    • 1
  • A. Paneque
    • 1
  1. 1.Institute de Bioquímica Vegetal y FotosíntesisUniuersidad de Sevilla-Consejo Superior de Inuestigaciones CientíficasSevillaSpain

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