Applied Biochemistry and Biotechnology

, Volume 30, Issue 1, pp 111–119 | Cite as

Rapid and simple isolation of multigram goat IgG from serum using avid aL™ and radial flow column

  • That T. Ngo
  • Neeta Khatter


Multigram quantities (2.5–10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL™ packed in a radialflow column. Avid AL™ is an affinity gel containing a synthetic, lowmol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.

Index Entries

Immunoglobulins affinity gels radial flow columns synthetic ligands goat serum 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Duhamel, R. C., Schur, P. H., Brendel, K., and Meezan, E. (1979),J. Immunol Methods 31, 211.CrossRefGoogle Scholar
  2. 2.
    Goding, J. W. (1969),Monoclonal Antibodies: Principles and Practice, p. 119.Google Scholar
  3. 3.
    Moellering, B. J., and Prior, C. P. (1990),Biopharm. 3, 34.Google Scholar
  4. 4.
    Fahnestock, S. R. (1987),Trends Biotechnol. 5, 79.CrossRefGoogle Scholar
  5. 5.
    Boyle, M. D. P. (1984),BioTechniques Nov./Dec, 334.Google Scholar
  6. 6.
    Mcguire, J. (1989),Am. Biotech. Lab. I, 28.Google Scholar
  7. 7.
    Porath, J., Maisano, F., and Belew, M., (1985),FEBS Letter 185, 306.CrossRefGoogle Scholar
  8. 8.
    Hutchens, T. W., and Porath, J. (1986),Anal. Biochem. 159, 217.CrossRefGoogle Scholar
  9. 9.
    Porath, J. (1987),Biopolymers 26, S193.CrossRefGoogle Scholar
  10. 10.
    Porath, J., and Belew, M. (1987),Trends Biotechnol. 5, 225.CrossRefGoogle Scholar
  11. 11.
    Belew, M., Junti, N., Larsson, A., and Porath, J. (1987),J. Immunol. Methods 102, 173.CrossRefGoogle Scholar
  12. 12.
    Nopper, B., Kohen, F., and Wilchek, M. (1989),Anal. Biochem. 180, 66.CrossRefGoogle Scholar
  13. 13.
    Ngo, T. T., and Khatter, N. (1990),J. Chromatog. 510, 281.CrossRefGoogle Scholar
  14. 14.
    Khatter, N., Matson, R. S., and Ngo, T. T. (1991),Am. Lab. in press.Google Scholar
  15. 15.
    Ngo, T. T. (1991), US Patent 4,981,961.Google Scholar

Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • That T. Ngo
    • 1
  • Neeta Khatter
    • 1
  1. 1.BioProbe International Inc.Tustin

Personalised recommendations