Rapid and simple isolation of multigram goat IgG from serum using avid aL™ and radial flow column
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Multigram quantities (2.5–10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL™ packed in a radialflow column. Avid AL™ is an affinity gel containing a synthetic, lowmol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.
Index EntriesImmunoglobulins affinity gels radial flow columns synthetic ligands goat serum
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