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Optimization of cellulase production by aspergillus niger NCIM 1207

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Abstract

Aspergillus niger NCIM 1207 produces high levels of extracellular β-glucosidase and xylanase activities in submerged fermentation. Among the nitrogen sources, ammonium sulfate, ammonium dihy-drogen orthophosphate, and corn-steep liquor were the best for the production of cellulolytic enzymes by A. niger. The optimum pH and temperature for cellulase production were 3.0-5.5 and 28°C, respectively. The cellulase complex of this strain was found to undergo catabolite repression in the presence of high concentrations of glucose. Glycerol at all concentrations caused catabolite repression of cellulase production. The addition of glucose (up to 1% concentration) enhanced the production of cellulolytic enzymes, but a higher concentration of glucose effected the pronounced repression of enzymes. Generally the growth on glucose- or glycerol-containing medium was accompanied by a sudden drop in the pH of the fermentation medium to 2.0.

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Authors and Affiliations

  1. NCIM, Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India

    D. V. Gokhale, S. G. Patil & K. B. Bastawde

Authors
  1. D. V. Gokhale
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  2. S. G. Patil
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  3. K. B. Bastawde
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Gokhale, D.V., Patil, S.G. & Bastawde, K.B. Optimization of cellulase production by aspergillus niger NCIM 1207. Appl Biochem Biotechnol 30, 99–109 (1991). https://doi.org/10.1007/BF02922026

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  • DOI: https://doi.org/10.1007/BF02922026

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