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Applied Biochemistry and Biotechnology

, Volume 44, Issue 1, pp 39–48 | Cite as

Some properties of a thermostable β-xylosidase fromRhodothermus marinus

  • Åsa Manelius
  • Leif Dahlberg
  • Olle Holst
Article

Abstract

The extracellular β-xylosidase (EC 3.2.1.37) excreted by the thermophilic eubacteriumRhodothermus marinus when grown on xylan has been investigated. The enzyme has been partially purified by ultrafiltration and gel filtration, and some of its characteristics are presented.Rhodothermus marinus grew on xylan with μmax= 0.4 h and the α-xylosidase activity was 50 nkat/mL after 24 h in a batch fermentation. The α-xylosidase activity had a half-life of more than 1 h at 90°C and of 14 h at 85 °C. At 80°C, 80% of the initial activity remained after 24 h. The initial activity increased with increasing temperature, showing maximal activity at 90°C. The β-xylosidase had a pH-optimum of 6 and was stable in the range between pH 5 and 9. At pH 10 and 11, 82 and 66%, respectively, of the initial activity remained after 24 h when incubated at 65°C. The molecular weight was estimated to be 169,000 dalton by gelfiltration.

Index Entries

Rhodothermus marinus β-xylosidase xylan degradation thermophilic microorganism thermostable enzyme 

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Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Åsa Manelius
    • 1
  • Leif Dahlberg
    • 1
  • Olle Holst
    • 1
  1. 1.Department of Biotechnology, Chemical CenterLund UniversityLundSweden

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