Applied Biochemistry and Biotechnology

, Volume 31, Issue 3, pp 247–251 | Cite as

Urease purification from the seeds ofCajanus Cajan and its application in a biosensor construction

  • P. C. Pandey
  • Vivek Pandey


Urease has been purified from the seeds ofCajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.

Index Entries

Enzyme purification enzyme electrode biosensor 


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Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • P. C. Pandey
    • 1
  • Vivek Pandey
    • 1
  1. 1.Department of ChemistryBanaras Hindu UniversityVaranasiIndia

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