Urease purification from the seeds ofCajanus Cajan and its application in a biosensor construction
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Urease has been purified from the seeds ofCajanus Cajan. The purification process involves three solvent extraction steps followed by DEAE-cellulose column chromatography. The specific activity of the purified enzyme is found to be 1920 U/mg with the recovery of 8%. The application of the purified enzyme in a biosensor construction is discussed.
Index EntriesEnzyme purification enzyme electrode biosensor
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