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Interaction of lectins withYersinia pestis strains

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Abstract

The ability of lectins to interact withYersinia pestis strains isolated from rodent fleas and human biological fluids, obtained from different geographic areas, was examined. Lectins ofCanavalia ensiformis, Ulex europaeus, Phaseolus vulgaris, andTriticum vulgaris, as well as a new autochthonous lectin ofSwartzia pickellii of undefined specificity, were used. Most of theY. pestis strains did not agglutinate withU. europaeus orC. ensiformis lectin. However, P.vulgaris lectin agglutinated suspensions of all the bacillus strains used. Fifteen of the 19 strains tested positive for assays usingS. pickellii lectin. It is believed this is the first report ofY. pestis strain agglutination by lectins. A similar agglutination pattern was obtained for lectins with specificity for oligosaccharides containingN-acetylglucosamine andS. pickellii lectin, which did bind to the affinity matrix chitin, a polysaccharide ofN-acetylglucosamine. The use of bacterial strains and commercial lectins of defined specificity may be an approach to providing evidence about the lectin binding sites of undefined monosaccharide specificity.

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References

  1. Devignat, R. (1951),Bull. WHO 4, 247.

    CAS  Google Scholar 

  2. Hudson, B. W., Quart, T. J., Sites, V. R., and Marshall, J. D. (1973),Am. J. Trop. Med. Hyg. 22, 642–643.

    CAS  Google Scholar 

  3. Hudson, B. W. and Quan, T. J. (1975),Am. J. Trop. Med. Hyg. 24, 968–973.

    CAS  Google Scholar 

  4. Hudson, B. W., Quan, T. J., and Bailey, B. E. (1976),Syst. Bacteriol. 26, 1–16.

    Article  Google Scholar 

  5. Almeida, A. M. P., Brasil, D. P., Carvalho, F. G., and Almeida, C. R. (1985),Rev. Inst. Med. Trop. Sāo Paulo,27, 207–218.

    Google Scholar 

  6. Goldstein, I. J., Hughes, R. C., Monsigny, M., Osawa, T., and Sharon, N. (1980),Nature 285, 66.

    Article  Google Scholar 

  7. Goldstein, I. J. and Hayes, C. E. (1978),Adv. Carbohydr. Chem. Biochem. 35, 127–340.

    Article  CAS  Google Scholar 

  8. Lis, H. and Sharon, N. (1986),Ann. Rev. Biochem. 35, 35–67.

    Article  Google Scholar 

  9. Doyle, R. J., Nedjat-Haiem, F., Keller, K. F., and Frasch, C. E. (1984),J. Clin. Microbiol. 19, 383–387.

    CAS  Google Scholar 

  10. Cole, H. B., Ezzell, J. W., Keller, K. F., and Doyle, R. J. (1984),J. Clin. Microbiol. 19, 48–53.

    CAS  Google Scholar 

  11. Schaefer, R. L., Keller, K. F., and Doyle, R. J. (1979),J. Clin. Microbiol. 10, 669–672.

    CAS  Google Scholar 

  12. Pistole, T. G. (1981),Annu. Rev. Microbiol. 35, 85–112.

    Article  CAS  Google Scholar 

  13. Bahmanyar, M. and Cavanaugh, D. C. (1976),Plague Manual. WHO, Geneva.

    Google Scholar 

  14. Baltazard, M., Davis, D. H. S., Devignat, R., Girard, G., Gohar, M. A., Kartman, L., Meyer, K. F., Parker, M. T., Pollitzer, M. T. R., Prince, F. M., Quan, S. F., and Wagle, P. (1956),Bull. WHO14, 457–509.

    CAS  Google Scholar 

  15. Baltazard, M. (1959),Bull. Acad. Natl. Med. 143, 517–522.

    CAS  Google Scholar 

  16. Baltazard, M. (1968),Rev. Bras. Malariol. Doencas Trop. 20, 335–336.

    CAS  Google Scholar 

  17. Karimi, Y. (1978),Bull. Soc. Path. Exot. 71, 45–48.

    CAS  Google Scholar 

  18. Bier, O. Bacteriologia e Imunologia. 20 ed. (1980), Melhoramentos,Sāo Paulo, pp. 783, 784.

    Google Scholar 

  19. Biochemical, and Organic Compounds for Research and Diagnostic Clinical Reagents (1987), Sigma, St. Louis, pp. 808–839.

  20. Yamashita, K., Hitoi, A., and Kobata, A. (1983),J. Biol. Chem. 258, 14753–14755.

    CAS  Google Scholar 

  21. Ohtani, K., Shibata, S., and Misaki, A. (1980),J. Biochem. 87, 407–416.

    CAS  Google Scholar 

  22. Bing, D. H., Weyand, J. G. M., and Stavitsky, A. B. (1967),Proc. Soc. Exp. Biol. Med. 124, 1166–1170.

    CAS  Google Scholar 

  23. Yajko, D. M., Chu, A., and Hadley, W. K. (1984),J. Clin. Microbiol. 19, 380–382.

    CAS  Google Scholar 

  24. Glosnicka, R. and Gruszkiewicz, E. (1980),Infect. Immun. 30, 506–512.

    CAS  Google Scholar 

  25. Bakharkh, E. E., Korobkova, I. E., and Shalaeva, A. F. (1958) Reported by R. W. Brubaker (1972),Curr. Top. Microbiol. Immunol. 57, 111–158.

    Google Scholar 

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Authors and Affiliations

  1. Departmento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco(UFPE), 50730, PE, Brazil

    Maria S. M. Cavalcanti & Luana C. B. B. Coelho

  2. Laboratório de Peste, Centro de Pesquisas Aggeu Magalhães(FIOCRUZ), 50730, Recife, PE, Brasil

    Maria S. M. Cavalcanti, Alzira M. P. Almeida & Luana C. B. B. Coelho

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  1. Maria S. M. Cavalcanti
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  2. Alzira M. P. Almeida
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  3. Luana C. B. B. Coelho
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Cavalcanti, M.S.M., Almeida, A.M.P. & Coelho, L.C.B.B. Interaction of lectins withYersinia pestis strains. Appl Biochem Biotechnol 26, 125–131 (1990). https://doi.org/10.1007/BF02921528

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  • DOI: https://doi.org/10.1007/BF02921528

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