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Applied Biochemistry and Biotechnology

, Volume 26, Issue 2, pp 125–131 | Cite as

Interaction of lectins withYersinia pestis strains

  • Maria S. M. Cavalcanti
  • Alzira M. P. Almeida
  • Luana C. B. B. Coelho
Article

Abstract

The ability of lectins to interact withYersinia pestis strains isolated from rodent fleas and human biological fluids, obtained from different geographic areas, was examined. Lectins ofCanavalia ensiformis, Ulex europaeus, Phaseolus vulgaris, andTriticum vulgaris, as well as a new autochthonous lectin ofSwartzia pickellii of undefined specificity, were used. Most of theY. pestis strains did not agglutinate withU. europaeus orC. ensiformis lectin. However, P.vulgaris lectin agglutinated suspensions of all the bacillus strains used. Fifteen of the 19 strains tested positive for assays usingS. pickellii lectin. It is believed this is the first report ofY. pestis strain agglutination by lectins. A similar agglutination pattern was obtained for lectins with specificity for oligosaccharides containingN-acetylglucosamine andS. pickellii lectin, which did bind to the affinity matrix chitin, a polysaccharide ofN-acetylglucosamine. The use of bacterial strains and commercial lectins of defined specificity may be an approach to providing evidence about the lectin binding sites of undefined monosaccharide specificity.

Index Entries

Lectins yersinia pestis 

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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Maria S. M. Cavalcanti
    • 1
    • 2
  • Alzira M. P. Almeida
    • 2
  • Luana C. B. B. Coelho
    • 1
    • 2
  1. 1.Departmento de Bioquímica, Centro de Ciências BiológicasUniversidade Federal de Pernambuco(UFPE)Brazil
  2. 2.Laboratório de PesteCentro de Pesquisas Aggeu Magalhães(FIOCRUZ)RecifeBrasil

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