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Conversion of ammonia or urea into essential amino acids,L-leucine,L-valine, andL-isoleucine using artificial cells containing an immobilized multienzyme system and dextran-NAD+

L-lactic dehydrogenase for coenzyme recycling

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Abstract

A multienzyme system consisting of leucine dehydrogenase (EC 1.4.1.9), L-lactic dehydrogenase (EC 1.1.1.27), urease (EC 3.5.1.5), and dextran-NAD+ was microencapsulated within artificial cells. This system could convert ammonia and urea into essential amino acids,L-leucine,L-valine, andL-isoleucine.L-lactate acted as a cosubstrate for the regeneration of dextran-NADH. Greater concentrations of L-lactate favored the higher conversion ratios. The effects of ammonium salts and urea on reaction rate were also studied. The relative reaction rates in ammonium salts solutions were 44.6–78.8% of those in urea solutions. More than 90% of the original activity was retained when artificial cells were kept at 4°C for 6 wk.

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Authors and Affiliations

  1. Artificial Cells and Organs Research Centre, McGill University, 3655 Drummond Street, H3G 1Y6, Montreal, Quebec, Canada

    Kang Fu Gu & Thomas Ming Swi Chang

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  1. Kang Fu Gu
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  2. Thomas Ming Swi Chang
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Gu, K.F., Ming Swi Chang, T. Conversion of ammonia or urea into essential amino acids,L-leucine,L-valine, andL-isoleucine using artificial cells containing an immobilized multienzyme system and dextran-NAD+ . Appl Biochem Biotechnol 26, 115–124 (1990). https://doi.org/10.1007/BF02921527

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  • DOI: https://doi.org/10.1007/BF02921527

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