Abstract
Pseudomonas pictorwn in free or immobilized form can deplete serum cholesterol. The reaction kinetics control the global rate of cholesterol depletion during the initial part of free P.pictorum fermentation. Then, mass transfer takes control of the global rate. In this part of the fermentation, the global rate is first order with respect to the cholesterol,concentration. The halftime was 330 min. The global rate is zero order with respect to the bacterial concentration. The activation energy was 83 kJ/mol. These results are consistent with the aqueous cholesterol diffusion model. The open pore agar microcapsule experimental effective diffusivity for lipoproteins at 37°C was 6.7xl0-11 m2/s. Mass transfer across the microcapsule was not the limiting factor for the global rate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gibbons, G. F., Mitropoulos, K. A., and Myart, N. B. (1982),Biochemistry of Cholesterol, Elsevier Biomedical Press, New York.
Brown, M. S. and Goldstein, J. L. (1983),Ann. Rev. Biochem. 52, 223–261.
Canadian Consensus Conference On Cholesterol: Final Report. (1988),CMAJ suppl. 139, 1–8.
Brown, R. L. and Peterson, G. E. (1966),J. Gen. Microbiol. 45, 441–450.
Arima, K., Nagasawa, M., Bae, M., and Tamura, G. (1969),Agr. Biol. Chem. 33, 1636–1643.
Owen, R. W., Mason, A. N., and Bliton, R. F. (1983),J.Lipid. Res. 24, 1500–1511.
Voets, J. P. and Lamot, E. (1974),Zeitschift All. Mikr. 14(1), 78,79.
Geier, M. R. and LaPolla, R. J. (1974),Biochem. Med. 11, 290–294.
Garofalo, F. A. (1987),Removal of Cholesterol by Pseudomonas pictorum, M. Eng. Thesis, McGill University, Montreal, Canada.
Garofalo, F. A. and Chang, T. M. S. (1989),Biomater. Artif. Cells Artif. Organs 17(3), 271–290.
Garofalo, F. A., and Chang, T. M. S. (1989),Biotech. Appl. Biochem. (submitted).
Hill, C. G. Jr. (1977),An Introduction to Chemical Engineering Kinetics & Reactor Design, John Wiley and Sons, New York, pp. 447–452.
Ollis, D. F. (1983),Ann. N. Y. Acad. Sci. 413, 144–156.
Crank, J. (1975),The Mathematics of Diffusion, Clarendon Press, oxford, pp. 93–96.
Phillips, M. C., Johnson, W. J., and Rothblat, G. H. (1987),Biochim. Biophys. Ada 906, 223–276.
Kao, Y. J., Doody, M. C., Smith, L. C. (1986),J. Lipid Res. 27, 781–785.
Quarfordt, S. H. and Hilderman, H. L. (1970),J. Lipid Res. 11, 528–535.
Bjornson, L. K., Gniewkowski, C., and Kayden, H. J. (1975),J. Lipid Res. 16, 39–53.
Gurd, F. R. N. (1960, inLipide Chemistry, Hanahan, D. J., ed., John Wiley and Sons, New York, p. 283.
Hagerman, J. S. and Gould, R. G. (1951),Proc. Soc. Exp. Biol. Med. 78, 329–332.
McLean, L. R. and Phillips, M. C. (1981),Biochemistry 20, 2893–2900.
Frederickson, D. S. (1967), inAtheroschlerosis Vascular Disease, Brest, A. N. Jr. and Moyer, J. H., eds., Appleton-Centrury-Crofts, New York, p. 158.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Garofalo, F.A., Chang, T.M.S. Effects of mass transfer and reaction kinetics on serum cholesterol depletion rates of free and immobilizedPseudomonas pictorwn . Appl Biochem Biotechnol 27, 75–91 (1991). https://doi.org/10.1007/BF02921517
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02921517