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Applied Biochemistry and Biotechnology

, Volume 27, Issue 1, pp 1–8 | Cite as

Kinetics of growth and metabolism ofClostridium thermosaccharolyticum culture

Isolation and characteristics of its plasmids
  • N. G. Belogurova
  • T. P. Mosolova
  • S. V. Kalyuzhnyy
  • S. D. Varfolomeyev
Article

Abstract

The kinetics of growth and metabolism ofClostridium thermo saccharolyticum DSM 571 has been studied at varying initial pH and glu cose concentration. A weak inhibitory effect of excess glucose on the specific growth rate has been shown. The effect of antibiotics of various classes on culture growth and hydrogen evolution has been studied. Streptomycin and kanamycin resistance of this culture has been re vealed as well as the phenomenon of increased hydrogen production in the presence of the above antibiotics. New plasmids, pNBl (4.9 kb) and pNB2 (2.0 kb), were isolated fromC. thermosaccharolyticum DSM 571. The restriction analysis of pNBl and pNB2 has been performed.

Index Entries

Clostridium thermosaccharolyticum glucose hydrogen ethanol acetic propionic and lactic acids carbon dioxide kinetics specific growth rate half-saturation constant inhibition constant antibiotic-resistance streptomycin kanamycin plasmid restriction analysis 

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Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • N. G. Belogurova
    • 1
  • T. P. Mosolova
    • 1
  • S. V. Kalyuzhnyy
    • 1
  • S. D. Varfolomeyev
    • 1
  1. 1.Division of Chemical Enzymology, Chemistry DepartmentMoscow State UniversityMoscowUSSR

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