Applied Biochemistry and Biotechnology

, Volume 27, Issue 1, pp 1–8 | Cite as

Kinetics of growth and metabolism ofClostridium thermosaccharolyticum culture

Isolation and characteristics of its plasmids
  • N. G. Belogurova
  • T. P. Mosolova
  • S. V. Kalyuzhnyy
  • S. D. Varfolomeyev


The kinetics of growth and metabolism ofClostridium thermo saccharolyticum DSM 571 has been studied at varying initial pH and glu cose concentration. A weak inhibitory effect of excess glucose on the specific growth rate has been shown. The effect of antibiotics of various classes on culture growth and hydrogen evolution has been studied. Streptomycin and kanamycin resistance of this culture has been re vealed as well as the phenomenon of increased hydrogen production in the presence of the above antibiotics. New plasmids, pNBl (4.9 kb) and pNB2 (2.0 kb), were isolated fromC. thermosaccharolyticum DSM 571. The restriction analysis of pNBl and pNB2 has been performed.

Index Entries

Clostridium thermosaccharolyticum glucose hydrogen ethanol acetic propionic and lactic acids carbon dioxide kinetics specific growth rate half-saturation constant inhibition constant antibiotic-resistance streptomycin kanamycin plasmid restriction analysis 


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  1. 1.
    Hoshino, T. and Ikeda, T. (1985),Can. J. Microbiol.3, 614.CrossRefGoogle Scholar
  2. 2.
    Imanaka, T., Ano, T., Fuji, M., and Aiba, S. (1981),J. Bacteriol.146, 1091.Google Scholar
  3. 3.
    Vasquer, C, Villanueva, J., and Vicuna, R. (1983),FEBS Lett.138, 339.CrossRefGoogle Scholar
  4. 4.
    McClung, L. S. (1935),J. Bacteriol.29, 189.Google Scholar
  5. 5.
    Zierhut, G., Piepersberg, W., and Bock, A. (1979),Eur. J. Biochem.98, 577.CrossRefGoogle Scholar
  6. 6.
    Zeikus, J. G., and Ng, T. K. (1982),Ann. Reps. Ferm. Proc.5, 263.Google Scholar
  7. 7.
    Wiegel, I., Ljungdahl, L. G., and Rawson, J. R. (1979),J. Bacteriol. 138, 800.Google Scholar
  8. 8.
    Matsumura, M., Katakura, Y., Imanaka, T., and Aiba, S. (1984),J. Bacteriol.160, 413.Google Scholar
  9. 9.
    Misra, A. K., Bose, N. K., and Johnson, J. (1988),Appl. Biochem. Biotechnol.17, 319.CrossRefGoogle Scholar
  10. 10.
    Lee, C.-K., Durre, P., Hippe, H., and Gottschalk, G. (1987),Arch. Microbiol.148, 107.CrossRefGoogle Scholar
  11. 11.
    Truffant, N., and Sebald, M. (1983),Mol. Gen. Genet.189, 178.CrossRefGoogle Scholar
  12. 12.
    Odelson, D. A., Rasmussen, L. J., Smith, C. J., and Macrina, F. L. (1987),Plasmid 17, 87.CrossRefGoogle Scholar
  13. 13.
    Varfolomeyev, S. D., and Kalyuzhnyy, S. V. (1989),Appl. Biochem. Biotech nol. 22, 331.CrossRefGoogle Scholar
  14. 14.
    Fechina, V. A., Gazarian, I. G., and Yegorov, A. M. (1988),Materialy 6 Vsesoyuznogo simposiuma “Inzhenernaya enzimologia” (Proceedings of the 6th All-Union Simposium “Enzyme Engineering”, Vilnus, v. 1, p. 155.Google Scholar
  15. 15.
    Berezin, I. V., Rabinovich, M. L., and Sinitsin, A. P. (1977),Biokhimia 9, 1631.Google Scholar
  16. 16.
    Gorina, I. A. and Yakovleva, V. I. (1980),Prikladn. Biokhim. Mikrobiol. (Appl. Biochem. and Microbiology) 16, 936.Google Scholar
  17. 17.
    Maniatis, T., Fritsch, E., and Sambrook, J. (1982),Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, N.Y., pp. 89–92.Google Scholar
  18. 18.
    Birnboim, H. C, and Doly, J. (1979),Nucl. Acids Res.7, 1513.CrossRefGoogle Scholar
  19. 19.
    Varfolomeyev, S. D., and Kalyuzhnyy, S. V. (1990),Kineticheskive osnovy mikrobiologicheskikh protsessov. (Kinetic grounds of microbiological processes), Vysshaya Shkola Press, Moscow, in press.Google Scholar

Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • N. G. Belogurova
    • 1
  • T. P. Mosolova
    • 1
  • S. V. Kalyuzhnyy
    • 1
  • S. D. Varfolomeyev
    • 1
  1. 1.Division of Chemical Enzymology, Chemistry DepartmentMoscow State UniversityMoscowUSSR

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