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Performance characteristics of Mammalian Cell Culture process operating continuously with protein-free medium

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Abstract

In this communication we briefly describe a continuous industrial process for the production of Tissue Plasminogen Activator (tPA), operating with a standard, basal medium (DME/F12). Process data of two culture runs has been chosen to present a performance profile over a production period of more than 3 mo. Developmental aspects of glucose utilization (as a process parameter) are briefly discussed.

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References

  1. Katinger, H. (1987),Develop. Biol. Stand. 66, 195–209.

    CAS  Google Scholar 

  2. Boraston, R., Lambert, K., and Birch, J. R. (1987),Develop. Biol. Stand. 66, 566.

    Google Scholar 

  3. Tovey, M. G. (1980),Adv. Cancer Res. 33, 1–36.

    Article  CAS  Google Scholar 

  4. Pullen, K. F., Johnson, M. D., Phillips, A. W., Ball, G. D., and Finter, N. B. (1985),Develop. Biol. Stand. 60, 175–177.

    CAS  Google Scholar 

  5. Mizrahi, A. and Lazar, A. (1988),Cytotechnology 1, 199–214.

    Google Scholar 

  6. Ham, R. G. and McKeehan, W. L. (1979),Methods in Enzymology, vol. 58, Jakoby, W. B. and Pastan, I. H., eds., Academic, London, pp. 44–93.

    Google Scholar 

  7. Wilson, E. (23.12.1983). European Patent Application # 0113319.

  8. Brown, P. C., Figueroa, C., Costello, M. A., Oakley, R., and Maciukas, S. M. (1988),Animal Cell Biotechnology, vol. 3, Spier, R. E. and Griffiths, J. B., eds., Academic, London.

    Google Scholar 

  9. Cole, S. P. C., Vreeken, E. H., Mirski, S. E. L., and Campling, B. G. (1987),J. Immunol. Methods. 97 29–35.

    Article  CAS  Google Scholar 

  10. Whiteside, J. P. and Spier, R. E. (1985),Dev. Biol. Stand. 60, 305–311.

    CAS  Google Scholar 

  11. Griffiths, J. B., Cameron, D. R., and Looby, D. (1987),Dev. Biol. Stand. 60, 331–338.

    Google Scholar 

  12. Levenspiel, O. (1972), Chemical Reaction Engineering, 2nd edition, John Wiley and Sons, New York, pp. 272–290.

    Google Scholar 

  13. Heussen, C., Joubert, F., and Dowdle, E. D. (1984),J. Biol. Chem. 259, 11635–11638.

    CAS  Google Scholar 

  14. Leist, C., Meyer, H.-P., and Feichter, A. (1986),J. Biotechn. 4, 235–246.

    Article  CAS  Google Scholar 

  15. Brosemer, R. W. and Rutter, W. J. (1961),Exp. Cell Res. 25, 101–113.

    Article  CAS  Google Scholar 

  16. S. Graff and K. S. McCarty (1954),Canc. Res. 18, No. 7, pp. 741–746.

    Google Scholar 

  17. Reuveny, S., Velez, D., Miller, L., and Macmillan, J. D. (1986),J. Immunol. Meth. 86, 61–69.

    Article  CAS  Google Scholar 

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Author notes

  1. R. Bliem, R. Oakley, K. Matsuoka & V. Taiariol

    Present address: Bristol-Myers Squibb Co., PO Box 4755, Syracuse, NY

Authors and Affiliations

  1. Bio-Response, Inc., 1978 West Winton Avenue, 94545, Hayward, CA

    R. Bliem, R. Oakley, K. Matsuoka & V. Taiariol

Authors
  1. R. Bliem
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  2. R. Oakley
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  3. K. Matsuoka
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  4. V. Taiariol
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Bliem, R., Oakley, R., Matsuoka, K. et al. Performance characteristics of Mammalian Cell Culture process operating continuously with protein-free medium. Appl Biochem Biotechnol 26, 217–229 (1990). https://doi.org/10.1007/BF02921503

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  • DOI: https://doi.org/10.1007/BF02921503

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