Abstract
Lysozyme was immobilized by two different methods in two different ways in order to obtain a preparation with an activity as high as possible toward a macromolecular substrate. The enzyme was bound as a Schiff base to a silicate carrier by using oxidized dextrans of different lengths as spacer and also was bound to controlled pore aminoglass via pyridino-4-aldehyde and BrCN. The latter preparations had activities up to 2.5% of the free lysozyme.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Venkatasubramanian, K., Vieth, W. R., and Wang, S.S. (1972),Hakko Kogaku Zasshi 50, 600–614.
Hailing, P. J., Asenjo, J. A., and Dunnil, P. (1979),Biotechnol. Bioeng. 21, 2359–2363.
Datta, R. and Ollis, D. F. (1974),Adv. Exp. Med. Biol. 42, 293–315.
Datta, R., Arminger, W., and Ollis, D. F. (1973),Biotechnol. Bioeng. 15, 993–1006.
Cherkasov, I. A., Kavchenko, N. A., Pavlovskii, P. E., and Bragina, L. P. (1975),Bioorg. Khim. 1, 50–55.
Cherkasov, I. A., Kravchenko, N. A., Pavlovskii, P. E., and Gravova, V. V. (1976),Bioorg. Khim. 2, 1422–1428.
Bartling, G. J., Brown, H. D., and Chattopadhyay, S. K. (1973),Nature (London)243, 342–344.
Brown, H. D. and Chattopadhyay, S. K. (1976),Methods in Enzymology vol. 44 (Mosbach, K., ed.), Academic, London, pp. 288–290.
Price, A. R. and Pethig, R. (1986)Biochim. Biophys. Acta 889, 128–135.
Maurel, P. and Douzou, P. (1976),J. Mol. Biol. 102, 253–264.
Shugar, D. (1952),Biochim. Biophys. Acta 8, 302–309.
Miller, L.M. and Golder, R. H. (1950),Arch. Bioch. Bioph. 29, 420–423.
Weisner, B. (1984),Methods of Enzymatic Analysis vol. 4 (Bergmeyer, H. U., ed.), Verlag Chemie, Weinheim, pp. 189–195.
Jacobs, S. (1959),Nature 183, 262.
Weetall, H. (1976),Methods in Enzymology, vol. 44 (Mosbach, K., ed.), Academic, London, pp. 139–140.
Miron, T. and Wilchek, M. (1982),J. Chromatography 215, 55–63.
Pittner, F., Miron, T., Pittner, G., and Wilchek, M. (1980),J. Solid Phase Biochem. 5, 147–166.
Goldstein, L. (1976), Methods in Enzymology, vol. 44 (Mosbach, K., ed.), Academic, London, pp. 397–450.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Moser, I., Dworsky, P. & Pittner, F. Degradation of bacterial cell walls by immobilized lysozyme. Appl Biochem Biotechnol 19, 243–249 (1988). https://doi.org/10.1007/BF02921496
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02921496