Immobilization/stabilization of lipase fromCandida rugosa
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With the aim of fixing the enzyme to the maxtrix by multiple covalent linkages, lipase fromCandida rugosa (formerlycylindracea) has been insolubilized through its amino groups on Sepharose 6B previously activated with 2,3-epoxy-1-propanol. Two main variables that are known to control the number of bonds formed have been tested: the contact time between enzyme and activated support, and the temperature at which the immobilization reaction is carried out. Studies on activity and stability of the different derivatives prepared showed that higher temperatures and longer contact times lead to insolubilized enzymes that are more resistant to inactivation by temperature and the presence of organic solvents. At 50°C and pH 7.2, the insoluble lipase was found to be 140 times more stable than its soluble counterpart.
Index EntriesGlycidol-activated agarose sepharose(aldehyde) lipase fromCandida cylindracea multipoint attachment, enzyme insolubilization by covalent lipase insolubilization activity-stability organic solvents, effect of
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- 1.Borgstrom, B. and Brockmam, H. L., eds. (1984),Upases, Elsevier, Amsterdam.Google Scholar
- 2.Macrae, A. R. and Hammond, R. C. (1985),Biotechnology and Genetic Engineering Reviews 3, 193.Google Scholar
- 5.Desnuelle, P. (1972),The Enzymes, 3rd ed., vol. 7, Boyer, P. D. ed., Academic, New York, pp. 575–616.Google Scholar
- 6.Benzonana, G. and Esposito, S. (1971),Biochim. Biophys. Acta 231, 15.Google Scholar
- 10.Guisán, J. M., Blanco, R. M., and Alvaro, G. (1987),Proc. 4th European Congress on Biotechnology, vol. 2, Neijssel, O. M., vanderMeer, R. R., and Luyben, K. Ch. A. M. eds., Elsevier, Amsterdam, pp. 97–100.Google Scholar
- 11.Blanco, R. M., Calvete, J. J., and Guisán, J. M. (1988),Enzyme Mic. Technol., in press.Google Scholar
- 13.Blanco, R. M. and Guisán, J. M. (1988),Enzyme Mic. Technol., in press.Google Scholar
- 14.Morrison, R. J. and Boyd, R. N. (1973),Organic Chemistry, Allyn and Bacon, Boston.Google Scholar
- 15.Anfinsen, C. B., Cuatrecasas, P., and Taniuchi, H. (1971),The Enzymes, vol. 4, Boyer, P. D., ed., Academic, New York, pp. 177–204.Google Scholar
- 16.Tomizuka, N., Ota, Y., and Yamada, K. (1966),Agr. Biol. Chem. 30, 576.Google Scholar
- 17.Tomizuka, N., Ota, Y., and Yamada, K. (1966),Agr. Biol Chem. 30, 1090.Google Scholar