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Endocrine Pathology

, 5:100 | Cite as

Carbonic anhydrases in the human adrenal gland and its disorders: Immunohistochemical and biochemical studies of the enzymes

  • Hironobu Sasano
  • Katsuaki Kato
  • Hiroshi Magura
  • Seppo Parkkila
  • Anna-Kaisa Parkkila
  • Hannu Rajaniemi
  • Naonori Sugai
Original Article

Abstract

To study the possible roles of carbonic anhydrases (CA) in the human adrenal glands, an immunohistochemical study (using polycloncal antibodies against CA I and II), and a biochemical assay of CA activity (based on the change in pH caused by the hydration of CO2) were performed in normal human adrenal tissue and adrenocortical adenomas. All of 10 normal adrenal glands showed positive staining for CA II in the zona glomerulosa, whereas weak CA I-positive staining was detected in only 5 glands. A biochemical study of 3 normal human adrenal specimens also demonstrated CA activity only in the outer adrenal cortex. Of 26 adrenocortical adenoma specimens ( 15 aldosteronomas, 6 nonfunctioning adenomas, and 5 Cushing’s adenomas), CA I and II immunoreactivity was observed in only 3 and 5 aldosteronoma samples, respectively. A biochemical analysis of CA in 5 adenoma specimens (3 aldosteronomas, 2 nonfunctioning adenomas) detected enzymatic activity in 2 aldosteronomas. In all 26 specimens of non-neoplastic attached adrenals of adrenocortical adenomas, CA II immunoreactivity was present in the zona glomerulosa cells. These results indicate that CAs are present in the zona glomerulosa cells of the non-neoplastic human adrenals glands (but not in the majority of adrenocortical adenomas) and that CA II may constitute the major form of the enzyme. Exclusive localization of CA II in the zona glomerulosa cells in human adrenal glands may suggest a possible involvement of CAs in aldosterone biosynthesis or secretion through ion transport, or both. Our data also suggest that CA II can be used as an immunochemical marker for the zona glomerulosa cells in human adrenal glands.

Keywords

Adenoma Aldosterone Carbonic Anhydrase Carbonic Anhydrase Activity Endocrine Pathology Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Crosby WH, Furth, FW. A modification of the benzidine method for measurement of hemoglobin in plasma and urine. Blood 2:380–383, 1956.Google Scholar
  2. 2.
    Dluhy RG, Axelrod L, Underwood RH, Williams GH. Studies of the control of plasma aldosterone concentration in normal man: II. Effect of dietary potassium and acute potassium infusion. J Clin Invest 51:1950–1957, 1972.PubMedCrossRefGoogle Scholar
  3. 3.
    Ehrlich EN. Electrolyte metabolism adrenocortical regulation of electrolytes and water metabolism. In: DeGroot LJ, ed.Endocrinology. Philadelphia: W. B. Saunders, 1989: 1582–1609.Google Scholar
  4. 4.
    Imai T, Seo H, Murata Y, Funahashi H, Satoh Y, Sasano H, Matui N, Takagi H. Dexamethasone-nonsuppressible cortisol in two cases with aldosterone producing adenoma. J Clin Endocrinol Metab 72:575- 581, 1985.Google Scholar
  5. 5.
    Lonnerholm G, Selking O, Wistrand PJ. Amount and distribution of carbonic anhydrases CA I and CA II in the gastrointestinal tract. Gastroenterology 88:1151–1161,1951.Google Scholar
  6. 6.
    Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1985.Google Scholar
  7. 7.
    Maren TH. A simplified micromethod for the determination of carbobic anhydrase and its inhibitors. J Pharmacol Exp Theor 130:26–29, 1960. 8. Maren TH. Carbonic anhydrase: chemistry, physiology and inhibition Physiol Rev 47:595-781, 1960.Google Scholar
  8. 9.
    Neville AM, O’Hare MJ. Human adrenal cortex. Berlin: Springer-Verlag, 1982.Google Scholar
  9. 10.
    Ogishima T, Suzuki H, Hata J, Mitani F, Ishimura Y. P450 and cytochrome P45011β in rat adrenal cortex: histochemical basis for the functional zonation. Endocrinology 130: 2971–2977, 1992.PubMedCrossRefGoogle Scholar
  10. 11.
    Parkkila AK, Parkkila S, Juronen T, Rajaniemi H. Carbonic anhydrase isoenzymes II and I are present in the zona glomerulosa cells of the human adrenal gland. Histochemistry 99:37–42, 1993.PubMedCrossRefGoogle Scholar
  11. 12.
    Parkkila S, Kaunisto K, Rajaniemi L, Kumpulainen T, Jokinen K, Rajaniemi H. Immunohistochemical localization of carbonic anhydrase isoenzymes VI, II and I in human parotid and submandibular glands. J Histochem Cytochem 38:941–947, 1990.PubMedGoogle Scholar
  12. 13.
    Puscas I, Buzas G, Piscas JC, Persa F. Carbonic anhydrase inhibitors: Antisecretory and cytoprotective properties. Acta Physiol Hungaria 73:167–177, 1989.Google Scholar
  13. 14.
    Sasano H, Mason JI, Sasano N. Immunohistochemical analysis of cytochrome P-450 17α-hydroxylase in pig adrenal cortex, testis and ovary. Mol Cell Endocrinol 62:197–202, 1987.Google Scholar
  14. 15.
    Sasano H, Mason JI, Sasano N, Nagura H. Immunolocalization of 3β-hydroxysteroid dehydrogenase in human adrenal cortex and in its disorders. Endocr Pathol 1:94–101, 1990.CrossRefGoogle Scholar
  15. 16.
    Sasano H, Fukushima K, Sasaki I, Matsuno S, Nagura H, Krozowski ZS. Immunolocalization of mineralocorticoid receptor in human kidney, pancreas, salivary, mammary and sweat glands: a light and electron microscopic immunohistochemical study. J Endocrinol 132:305–310, 1992.PubMedGoogle Scholar
  16. 17.
    Sasano N. Functional pathology in adrenal cortex. Trans Soc Pathol Jpn 63:31–54, 1975.Google Scholar
  17. 18.
    Shibata H, Ogishima T, Mitani F, Suzuki H, Murakami M, Saruta T, Ishimura Y. Regulation of aldosterone synthase cytochrome P-450 in rat adrenals by angiotensin II and potassium. Endocrinology 128:2534–2539, 1991.PubMedGoogle Scholar
  18. 19.
    Spicer SS, Sens MA, Tashian RE. Immunocutochemical demonstration of carbonic anhydrase in human epithelial cells. J Histochem Cytochem 30:864–873, 1982.PubMedGoogle Scholar
  19. 20.
    Suzuki S, Ren LJ, Chen H. Further studies on the effect of aldosterone on Mg2+-HCO3- ATPase and carbonic anhydrase from rat intestinal mucosa. J Steroid Biochem 33:89–99, 1989.PubMedCrossRefGoogle Scholar
  20. 21.
    Tait JF, Tait SAS. Recent perspectives on the history of the adrenal cortex. The Sir Henry Dale lecture for 1979. J Endocrinol 83:3–24, 1979.Google Scholar

Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Hironobu Sasano
    • 1
  • Katsuaki Kato
    • 1
  • Hiroshi Magura
    • 1
  • Seppo Parkkila
    • 2
  • Anna-Kaisa Parkkila
    • 2
  • Hannu Rajaniemi
    • 2
  • Naonori Sugai
    • 3
  1. 1.Department of PathologyTohoku University School of MedicineSendaiJapan
  2. 2.Department of AnatomyUniversity of OuluOuluFinland
  3. 3.Department of AnatomyFukushima Prefectural Medical CollegeFukushimaJapan

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