Advertisement

Endocrine Pathology

, 5:27 | Cite as

Localization of basic fibroblast growth factor (bFGFJ) protein and mRNA in human pituitaries: regulation of bFGF mRNA by gonadotropin-releasing hormone

  • Long Jin
  • William F. Chandler
  • Ricardo V. Lloyd
Original Article

Abstract

Distribution of basic fibroblast growth factor (bFGF) in non-neoplastic and adenomatous human pituitaries was analyzed by immunohistochemistry, in situ, and Northern hybridization analyses. Basic FGF protein and mRNA were present in most hormone-producing cells of the non-neoplastic pituitary and in all pituitary adenomas examined. Northern hybridization analysis revealed 7.3 kb and 3.7 kb transcripts, which were most abundant in PRL-and ACTH-secreting adenomas. Three null cell and 3 gonadotroph adenomas were cultured for 7 days in the presence of 10-7 mol/L GnRH. There was an increase (range, 0.4–1.8-fold based on mean grain counts after in situ hybridization) in bFGF in most adenomas (5 of 6). Northern analysis showed an increase in the 7.3-kb transcript in a null-cell adenoma. Chromogranin B mRNA was also increased by Northern analysis in the same neoplasm. These results indicate that bFGF protein and mRNA are widely distributed in non-neoplastic and adenomatous human pituitaries and that the mRNA levels of bFGF in null cell and gonadotroph adenomas can be modulated by GnRH, implicating hypothalamic peptides in regulating bFGF in the anterior pituitary.

Keywords

Adenoma Pituitary Adenoma Basic Fibroblast Growth Factor Null Cell Endocrine Pathology Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Baird A, Esch F, Mormede P, Ueno N, Ling N, Bohlen P, Ying S-Y, Wehrenberg WB, Guillenin R Molecular characterization of fibroblast growth factor: distribution and biological activities in various tissues. Recent Prog Horm Res 42:143–205, 1986.PubMedGoogle Scholar
  2. 2.
    Baird A, Mormede P, Ying SY, Wehrenberg WB, Ueno N, Ling N, Guillemin R. A nonmitogenic pituitary function of fibroblast growth factor: regulation of thyrotropin and prolactin secretion. Proc Natl Acad Sci USA 82:5545–5549, 1985.PubMedCrossRefGoogle Scholar
  3. 3.
    Beauchamp RD, Coffey RJ Jr, Lyons RM, Perkett EA, Townsend CM Jr, Moses HL. Human carcinoid cell production of paracrine growth factors that can stimulate fibroblast and endothelial cell growth. Cancer Res 51:5253–5260, 1991.PubMedGoogle Scholar
  4. 4.
    Black EG, Logan A, Davis JRE, Sheppard MC. Basic fibroblast growth factor affects DNA synthesis and cell function and activates multiple signalling pathways in rat thyroid FRTL-5 and pituitary GH3 cells. J Endocrinol 127:39–46, 1990.PubMedGoogle Scholar
  5. 5.
    Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:16–159, 1987.CrossRefGoogle Scholar
  6. 6.
    Ezzat S, Melmed S. The role of growth factors in the pituitary. J Endocrinol Invest 13:691–698, 1990.PubMedGoogle Scholar
  7. 7.
    Ferrara N, Schweigerer L, Neufeld G, Mitchell R, Gospodarowicz D. Pituitary follicular cells produce basic fibroblast growth factor. Proc Natl Acad Sci USA 84:5773–5777, 1987.PubMedCrossRefGoogle Scholar
  8. 8.
    Gonzalez AM, Frautschy SA, Reimers D, Ong M, Farris J, Baird A. Anterior and posterior pituitary synthesis of basic FGF and one of its receptors, FIG implication for an in vivo neuroendocrine role of basic fibroblast growth factor (FGF). Presented at the 74th Annual Meeting of the Endocrine Society, San Antonio, TX, June 24–27, 1992, p. 87.Google Scholar
  9. 9.
    Gospodarowicz D. Localization of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature 249:123–127, 1974.PubMedCrossRefGoogle Scholar
  10. 10.
    Gospodarowicz D, Ferrara N. Fibroblast growth factor and the control of pituitary and gonad development and function. J Steroid Biochem 32:183–191, 1989.PubMedCrossRefGoogle Scholar
  11. 11.
    Grothe C, Unsicker K. Immunocytochemical localization of basic fibroblast growth factor in bovine adrenal gland, ovary and pituitary. J Histochem Cytochem 37:1877–1883, 1989.PubMedGoogle Scholar
  12. 12.
    Halper J, Parnell PG, Carter BJ, Ren P, Scheithauer BW. Presence of growth factors in human pituitary. Lab Invest 66:639–645, 1992.PubMedGoogle Scholar
  13. 13.
    Inoue K, Sakai T, Hattorri M. The cell-adhesive effect of basic fibroblast growth factor on pituitary cellsin vitro. J Endocrinol 136:381–386, 1991.Google Scholar
  14. 14.
    Jin L, Chandler WF, Lloyd RV. Localization of specific growth factors (GF) on normal and adenomatous human pituitaries. Presented at the 74th Annual Meeting of the Endocrine Society, San Antonio, TX, June 24–27, 1992, p. 315.Google Scholar
  15. 15.
    Larson GH, Koos RD, Sortino MD, Wise PM. Acute effect of basic fibroblast growth factor on secretion of prolactin as assessed by the reverse hemolytic plaque assay. Endocrinology 126:927–932, 1990.PubMedGoogle Scholar
  16. 16.
    Li Y, Koga M, Kasayama S, Matsumoto K, Arita N, Hayakawa T, Sato B. Identification and characterization of high molecular weight forms of basic fibroblast growth factor in human pituitary adenomas. J Clin Endocrinol Metab 75:1436–1441, 1992.PubMedCrossRefGoogle Scholar
  17. 17.
    Mormede P, Baird A. Estrogens, cyclic adenosine 3′,5′-monophosphate, and phorbal esters modulate the prolactin response of GH3 cells to basic fibroblast growth factor. Endocrinology 122:2265–2271, 1988.PubMedGoogle Scholar
  18. 18.
    Presta M, Foiani M, Rusnati M, Joseph-Silverstein J, Maier JAM, Ragnotti G. High molecular weight immunoreactive basic fibroblast growth factor-like protein in rat pituitary and brain. Neurosci Lett 90:308–313, 1988.PubMedCrossRefGoogle Scholar
  19. 19.
    Prysor-Jones RA, Silverlight JJ, Jenkins JS. Oestradiol, vasoactive intestinal peptide and fibroblast growth factor in the growth of human pituitary tumor cells in vitro. J Endocrinol 120:171–177, 1989.PubMedGoogle Scholar
  20. 20.
    Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.Google Scholar
  21. 21.
    Schechter J, Weiner R. Changes in basic fibroblast growth factor coincident with estradiol-induced hyperplasia of the anterior pituitaries of Fischer 344 and Sprague-Dawley rats. Endocrinology 129:2400–2408, 1991.PubMedGoogle Scholar
  22. 22.
    Schweigerer L, Neufeld G, Friedman J, Abraham JA, Fiddes JC, Gasporodarowicz D. Capillary endothelial cells express basic fibroblast growth factor, a mitogen that promotes their own growth. Nature 325: 257–259, 1987.PubMedCrossRefGoogle Scholar
  23. 23.
    Schweigerer L, Neufeld G, Gospodarowicz D. Basic fibroblast growth factor as a growth inhibitor for cultured human tumor cells. J Clin Invest 80:1516–1520, 1987.PubMedCrossRefGoogle Scholar
  24. 24.
    Silverlight JJ, Prysor-Jones RA, Jenkins JS. Basic fibroblast growth factor in human pituitary tumor. Clin Endocrinol (Oxf) 32:669–676, 1990.CrossRefGoogle Scholar
  25. 25.
    Song JY, Jin L, Chandler WF, England BG, Smart JB, Landefeld TD, Lloyd RV. Gonadotropin releasing hormone regulates gonadotropin beta-subunit and chromogranin B mRNAs in cultured chromogranin A positive pituitary adenomas. J Clin Endocrinol Metab 71:622–630, 1990.PubMedGoogle Scholar
  26. 26.
    Vlodavsky I, Bar-Shavit R, Ishai-Michaeli R, Bashkin P, Fuks Z. Extracellular sequestration and release of fibroblast growth factor: a regulatory mechanism. Trends Biochem Sci 16:268–271, 1991.PubMedCrossRefGoogle Scholar
  27. 27.
    Webster J, Ham J, Bevan JS, Ten Horn CD, Scanlon MF. Preliminary characterization of growth factors secreted by human pituitary tumors. J Clin Endocrinol Metab 72:687–692, 1991.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Long Jin
    • 1
  • William F. Chandler
    • 2
  • Ricardo V. Lloyd
    • 1
  1. 1.Department of PathologyUniversity of Michigan Medical CenterAnn Arbor
  2. 2.Surgery Section of NeurosurgeryUniversity of Michigan Medical CenterAnn Arbor

Personalised recommendations