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Localization of basic fibroblast growth factor (bFGFJ) protein and mRNA in human pituitaries: regulation of bFGF mRNA by gonadotropin-releasing hormone

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Abstract

Distribution of basic fibroblast growth factor (bFGF) in non-neoplastic and adenomatous human pituitaries was analyzed by immunohistochemistry, in situ, and Northern hybridization analyses. Basic FGF protein and mRNA were present in most hormone-producing cells of the non-neoplastic pituitary and in all pituitary adenomas examined. Northern hybridization analysis revealed 7.3 kb and 3.7 kb transcripts, which were most abundant in PRL-and ACTH-secreting adenomas. Three null cell and 3 gonadotroph adenomas were cultured for 7 days in the presence of 10-7 mol/L GnRH. There was an increase (range, 0.4–1.8-fold based on mean grain counts after in situ hybridization) in bFGF in most adenomas (5 of 6). Northern analysis showed an increase in the 7.3-kb transcript in a null-cell adenoma. Chromogranin B mRNA was also increased by Northern analysis in the same neoplasm. These results indicate that bFGF protein and mRNA are widely distributed in non-neoplastic and adenomatous human pituitaries and that the mRNA levels of bFGF in null cell and gonadotroph adenomas can be modulated by GnRH, implicating hypothalamic peptides in regulating bFGF in the anterior pituitary.

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References

  1. Baird A, Esch F, Mormede P, Ueno N, Ling N, Bohlen P, Ying S-Y, Wehrenberg WB, Guillenin R Molecular characterization of fibroblast growth factor: distribution and biological activities in various tissues. Recent Prog Horm Res 42:143–205, 1986.

    CAS  PubMed  Google Scholar 

  2. Baird A, Mormede P, Ying SY, Wehrenberg WB, Ueno N, Ling N, Guillemin R. A nonmitogenic pituitary function of fibroblast growth factor: regulation of thyrotropin and prolactin secretion. Proc Natl Acad Sci USA 82:5545–5549, 1985.

    Article  CAS  Google Scholar 

  3. Beauchamp RD, Coffey RJ Jr, Lyons RM, Perkett EA, Townsend CM Jr, Moses HL. Human carcinoid cell production of paracrine growth factors that can stimulate fibroblast and endothelial cell growth. Cancer Res 51:5253–5260, 1991.

    CAS  PubMed  Google Scholar 

  4. Black EG, Logan A, Davis JRE, Sheppard MC. Basic fibroblast growth factor affects DNA synthesis and cell function and activates multiple signalling pathways in rat thyroid FRTL-5 and pituitary GH3 cells. J Endocrinol 127:39–46, 1990.

    Article  CAS  Google Scholar 

  5. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:16–159, 1987.

    Article  Google Scholar 

  6. Ezzat S, Melmed S. The role of growth factors in the pituitary. J Endocrinol Invest 13:691–698, 1990.

    Article  CAS  Google Scholar 

  7. Ferrara N, Schweigerer L, Neufeld G, Mitchell R, Gospodarowicz D. Pituitary follicular cells produce basic fibroblast growth factor. Proc Natl Acad Sci USA 84:5773–5777, 1987.

    Article  CAS  Google Scholar 

  8. Gonzalez AM, Frautschy SA, Reimers D, Ong M, Farris J, Baird A. Anterior and posterior pituitary synthesis of basic FGF and one of its receptors, FIG implication for an in vivo neuroendocrine role of basic fibroblast growth factor (FGF). Presented at the 74th Annual Meeting of the Endocrine Society, San Antonio, TX, June 24–27, 1992, p. 87.

  9. Gospodarowicz D. Localization of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature 249:123–127, 1974.

    Article  CAS  Google Scholar 

  10. Gospodarowicz D, Ferrara N. Fibroblast growth factor and the control of pituitary and gonad development and function. J Steroid Biochem 32:183–191, 1989.

    Article  CAS  Google Scholar 

  11. Grothe C, Unsicker K. Immunocytochemical localization of basic fibroblast growth factor in bovine adrenal gland, ovary and pituitary. J Histochem Cytochem 37:1877–1883, 1989.

    Article  CAS  Google Scholar 

  12. Halper J, Parnell PG, Carter BJ, Ren P, Scheithauer BW. Presence of growth factors in human pituitary. Lab Invest 66:639–645, 1992.

    CAS  PubMed  Google Scholar 

  13. Inoue K, Sakai T, Hattorri M. The cell-adhesive effect of basic fibroblast growth factor on pituitary cellsin vitro. J Endocrinol 136:381–386, 1991.

    Article  Google Scholar 

  14. Jin L, Chandler WF, Lloyd RV. Localization of specific growth factors (GF) on normal and adenomatous human pituitaries. Presented at the 74th Annual Meeting of the Endocrine Society, San Antonio, TX, June 24–27, 1992, p. 315.

  15. Larson GH, Koos RD, Sortino MD, Wise PM. Acute effect of basic fibroblast growth factor on secretion of prolactin as assessed by the reverse hemolytic plaque assay. Endocrinology 126:927–932, 1990.

    Article  CAS  Google Scholar 

  16. Li Y, Koga M, Kasayama S, Matsumoto K, Arita N, Hayakawa T, Sato B. Identification and characterization of high molecular weight forms of basic fibroblast growth factor in human pituitary adenomas. J Clin Endocrinol Metab 75:1436–1441, 1992.

    CAS  PubMed  Google Scholar 

  17. Mormede P, Baird A. Estrogens, cyclic adenosine 3′,5′-monophosphate, and phorbal esters modulate the prolactin response of GH3 cells to basic fibroblast growth factor. Endocrinology 122:2265–2271, 1988.

    Article  CAS  Google Scholar 

  18. Presta M, Foiani M, Rusnati M, Joseph-Silverstein J, Maier JAM, Ragnotti G. High molecular weight immunoreactive basic fibroblast growth factor-like protein in rat pituitary and brain. Neurosci Lett 90:308–313, 1988.

    Article  CAS  Google Scholar 

  19. Prysor-Jones RA, Silverlight JJ, Jenkins JS. Oestradiol, vasoactive intestinal peptide and fibroblast growth factor in the growth of human pituitary tumor cells in vitro. J Endocrinol 120:171–177, 1989.

    Article  CAS  Google Scholar 

  20. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.

    Google Scholar 

  21. Schechter J, Weiner R. Changes in basic fibroblast growth factor coincident with estradiol-induced hyperplasia of the anterior pituitaries of Fischer 344 and Sprague-Dawley rats. Endocrinology 129:2400–2408, 1991.

    Article  CAS  Google Scholar 

  22. Schweigerer L, Neufeld G, Friedman J, Abraham JA, Fiddes JC, Gasporodarowicz D. Capillary endothelial cells express basic fibroblast growth factor, a mitogen that promotes their own growth. Nature 325: 257–259, 1987.

    Article  CAS  Google Scholar 

  23. Schweigerer L, Neufeld G, Gospodarowicz D. Basic fibroblast growth factor as a growth inhibitor for cultured human tumor cells. J Clin Invest 80:1516–1520, 1987.

    Article  CAS  Google Scholar 

  24. Silverlight JJ, Prysor-Jones RA, Jenkins JS. Basic fibroblast growth factor in human pituitary tumor. Clin Endocrinol (Oxf) 32:669–676, 1990.

    Article  CAS  Google Scholar 

  25. Song JY, Jin L, Chandler WF, England BG, Smart JB, Landefeld TD, Lloyd RV. Gonadotropin releasing hormone regulates gonadotropin beta-subunit and chromogranin B mRNAs in cultured chromogranin A positive pituitary adenomas. J Clin Endocrinol Metab 71:622–630, 1990.

    Article  CAS  Google Scholar 

  26. Vlodavsky I, Bar-Shavit R, Ishai-Michaeli R, Bashkin P, Fuks Z. Extracellular sequestration and release of fibroblast growth factor: a regulatory mechanism. Trends Biochem Sci 16:268–271, 1991.

    Article  CAS  Google Scholar 

  27. Webster J, Ham J, Bevan JS, Ten Horn CD, Scanlon MF. Preliminary characterization of growth factors secreted by human pituitary tumors. J Clin Endocrinol Metab 72:687–692, 1991.

    Article  CAS  Google Scholar 

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Author notes

  1. Ricardo V. Lloyd

    Present address: Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Foundation, 200 First Street, SW, 55905, Rochester, MN

Authors and Affiliations

  1. Department of Pathology, University of Michigan Medical Center, Ann Arbor, MI

    Long Jin & Ricardo V. Lloyd

  2. Surgery Section of Neurosurgery, University of Michigan Medical Center, Ann Arbor, MI

    William F. Chandler

Authors
  1. Long Jin
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  2. William F. Chandler
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  3. Ricardo V. Lloyd
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Additional information

This work was supported in part by NIH Grant CA 42951.

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Jin, L., Chandler, W.F. & Lloyd, R.V. Localization of basic fibroblast growth factor (bFGFJ) protein and mRNA in human pituitaries: regulation of bFGF mRNA by gonadotropin-releasing hormone. Endocr Pathol 5, 27–34 (1994). https://doi.org/10.1007/BF02921367

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