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Hormone-like product of vascular tissue stimulating starch accumulation in pith explants of kale and endogenous cytokinins

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Biologia Plantarum

Abstract

When stem explants of kale (Brassica oleracea L. var.medullosa), containing pith parenchyma and a strip of vascular tissue, were cultured on simple sucrose medium, a hormone-like factor was transported from the vascular tissue to the adjacent pith, where it stimulated accumulation of starch. Similarly, up to a sevenfold increase of starch content in explants could be induced by cytokinins added to the culture medium. The relative stimulatory effect of several cytokinins (5×10−6 M) and hormone-like product of vascular tissue (HPVT) in a typical experiment were: control (1.0), trans-zeatin (6.7), HPVT (6.2), N6-[2-isopentenyl]adenine (5.4), transzeatin riboside (5.2), N6-[2-isopentenyl]adenosine (5.4), kinetin (3.6), 6-benzylaminopurine (3.5), and adenine (2.1). Concentration of endogenous cytokinins was determined using ELISA (trans-zeatin, N6-[2-isopentenyl]adenine and their ribosides) andAmaranthus bioassay (total cytokinins). No effect of vascular tissue on the level of endogenous cytokinins in explants was found. The results support the conclusions of previous experiments that the HPVT stimulating starch accumulation is not a cytokinin.

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Authors and Affiliations

  1. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic

    V. Řeřábek & A. Hájek

  2. Faculty of Natural Science, Charles University, Viničná 5, 128 44, Praha 2, Czech Republic

    J. Luštinec

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  1. V. Řeřábek
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  2. A. Hájek
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  3. J. Luštinec
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Communicated by I. MACHÁČKOVÁ

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Řeřábek, V., Hájek, A. & Luštinec, J. Hormone-like product of vascular tissue stimulating starch accumulation in pith explants of kale and endogenous cytokinins. Biol Plant 36, 59–64 (1994). https://doi.org/10.1007/BF02921270

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  • DOI: https://doi.org/10.1007/BF02921270

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