Biologia Plantarum

, Volume 36, Issue 1, pp 59–64 | Cite as

Hormone-like product of vascular tissue stimulating starch accumulation in pith explants of kale and endogenous cytokinins

  • V. Řeřábek
  • A. Hájek
  • J. Luštinec
Original Papers


When stem explants of kale (Brassica oleracea L. var.medullosa), containing pith parenchyma and a strip of vascular tissue, were cultured on simple sucrose medium, a hormone-like factor was transported from the vascular tissue to the adjacent pith, where it stimulated accumulation of starch. Similarly, up to a sevenfold increase of starch content in explants could be induced by cytokinins added to the culture medium. The relative stimulatory effect of several cytokinins (5×10−6 M) and hormone-like product of vascular tissue (HPVT) in a typical experiment were: control (1.0), trans-zeatin (6.7), HPVT (6.2), N6-[2-isopentenyl]adenine (5.4), transzeatin riboside (5.2), N6-[2-isopentenyl]adenosine (5.4), kinetin (3.6), 6-benzylaminopurine (3.5), and adenine (2.1). Concentration of endogenous cytokinins was determined using ELISA (trans-zeatin, N6-[2-isopentenyl]adenine and their ribosides) andAmaranthus bioassay (total cytokinins). No effect of vascular tissue on the level of endogenous cytokinins in explants was found. The results support the conclusions of previous experiments that the HPVT stimulating starch accumulation is not a cytokinin.


Vascular Tissue Brassica Oleracea Starch Accumulation Endogenous Cytokinin Cytokinin Level 
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Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • V. Řeřábek
    • 1
  • A. Hájek
    • 1
  • J. Luštinec
    • 2
  1. 1.Institute of Plant Molecular BiologyAcademy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic
  2. 2.Faculty of Natural ScienceCharles UniversityPraha 2Czech Republic

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