Biologia Plantarum

, Volume 36, Issue 1, pp 9–13 | Cite as

Isolation and culture of suspension-derived protoplasts ofBeta vulgaris L.

  • A. Majewska-Sawka
  • H. Nakashima
  • K. Mori
Original Papers


Sugar beet protoplasts (Beta vulgaris L.) were isolated from hypocotyl-derived suspension cells and cultured on modified Murashige and Skoog medium supplemented with 5 μM naphthaleneacetic acid (NAA) and 2 μM 6-benzyl-aminopurine (BAP). Protoplasts were plated at a density 1.0–1.5×105 cm−3 and incubated in either liquid medium or in medium solidified by 1.2% agarose, at 25°C in the dark. Comparison of two methods of culture unequivocally showed the second to be superior. Immobilizing the protoplast in agarose proved to be essential for obtaining sustained protoplast division and reproducible colony formation. The plating efficiency after two weeks of culture, expressed as the percentage of protoplasts which developed to form colonies, reached 40%. Subsequent subcultures of protoplast-derived callus to regeneration media with different concentrations of BAP (5 μM, 10 μM, 20 μM, 30 μM) resulted in very good callus proliferation at the three lowest concentrations, although organogenesis was not achieved.


Sugar Beet Cytoplasmic Male Sterility Protoplast Isolation Sugar Beet Genotype Beta Maritima 
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Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • A. Majewska-Sawka
    • 1
  • H. Nakashima
    • 2
  • K. Mori
    • 2
  1. 1.Institute for Plant Breeding and AcclimatizationBydgoszczPoland
  2. 2.Faculty of AgricultureHokkaido UniversitySapporoJapan

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