Abstract
The effects of several growth and trophic substances on bud and root neoformation on leaf fragments ofBegonia rex were studied in precisely defined environmental conditions. IAA, depending on the type of treatment, had different effects. In aseptic cultures, a notable stimulation of bud formation was observed at certain concentrations. However, non aseptic treatments of IAA had no visible effects except at very high concentrations.(10−3 M) where bud formation was totally inhibited and root formation was favored. NAA, at 10−6 M and 10−5 M strongly stimulated root formation and inhibited shoot formation. All the cytokinins used stimulated bud formation and inhibited partially or totally root formation. Gibberellic acid inhibited bud and root formation. Glucose and sucrose clearly stimulated bud and root formation and sucrose, when applied simultaneously with other growth substances, modified the effects of these substances alone. The most favorable environmental conditions were at 24°C in a 24 h photoperiod but other temperatures (17 to 27°C) and photoperiods (9 or 16 h) did not prevent neoformation.
Abstract
Vliv kyseliny indolyl-3-octové (IAA) na tvorbu pupenů a kořenů u listových fragmentůBegonia rex závisí na způsobu aplikace. V aseptické kultuře byla v jistých koncentracích pozorována značná stimulace tvorby pupenů. V neaseptické kultuře však IAA nemá viditelný vliv s výjimkou velmi vysokých koncentrací (10−3 M), v nichž je tvorba pupenů zcela inhibována a je podporována tvorba kořenů. Všechny použité cytokininy stimulují tvorbu pupenů a inhibují částečně nebo úplně tvorbu kořenů. Kyselina giberelová tvorbu pupenů i kořenů inhibuje. Glukosa a sacharosa zřetelně stimulují tvorbu pupenů i kořenů, sacharosa aplikovaná spolu s růstovými látkami jejich vliv modifikuje. Nejvhodnější je teplota 24°C při fotoperiodě 24 h, avšak jiné teploty (17 až 27°C) a fotoperiody (9 a 16 h) novotvoření nezabraňují.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Bibliographie
Arnason, A., Tran Thanh Van, M., Prevot, P.: Capacité formatrice de bourgeons chez les feuilles adultes deBegonia rex Putz.—Compt. rend. Acad. Sci. (Paris)264: 599–602, 1967.
Bigot, C.: Action de trois adénines substituées sur l'apparition de néoformations sur des explantats foliaires deBegonia.—Bull. Soc. bot. Fr.113: 433–439, 1966.
Chlyah-Arnason, A., Tran Thanh Van, M.: Budding capacity of undetachedBegonia rex leaves.—Nature218: 493, 1968.
Chlyah, A., Tran Thanh Van, M.: Comparison of the localization of nucleic acid synthesis during bud formation on leaf fragments and on intact undetached leaves.—Biol. Plant.13: 184–188, 1971.
Chouard, P.: Production expérimentale de bourgeons sous l'effet d'hétéroauxine.—Compt. rend. Acad. Sci. (Paris)206: 1401–1406, 1938.
Drira, A: Etude de la néoformation de méristèmes caulinaires chez les feuilles deNautilocalyx lynchei (Hook) et les altérations de la polarité par les facteurs de croissance.—Diplôme d'Études Approfondies, Paris 1968.
Hartsema, A.: Anatomische und experimentelle Untersuchungen über das Auftreten von Neubildungen an Blättern vonBegonia rex.—Rec. Trav. bot. Neerl.23, 306–361, 1926.
Heide, O. M.: Interaction of temperature, auxine and kinine on the regeneration ability ofBegonia rex leaf cuttings.—Physiol. Plant.18: 891–920, 1965.
Heide, O. M.: Non-reversibility of gibberellin-induced inhibition of regeneration inBegonia leaves.—Physiol. Plant.22: 671–679, 1969.
Hillman, W. S., Galston, A. W.: The effect of external factors on auxin content.—In:Ruhland, W. (ed.), Encyclopedia of Plant Physiology. Vol. 14. Pp. 683–702. Springer Verlag, Berlin 1961.
Key, J. L., Shannon, J. C.: Enhancement by auxin of RNA synthesis in excised soybean hypocotyl tissue.—Plant Physiol.39: 360–364, 1964.
Madison, J. T., Everett, G. A., Kung, H. K.: Oligosides from yeast tyrosine transfer ribonucleic acid.—J. biol. Chem.242: 1318–1323, 1967.
Masuda, Y.: Auxin-induced growth of tuber tissue of Jerusalem artichoke. III. Effect of actinomycin D on auxin-induced expansion and RNA synthesis.—Plant Cell Physiol.7: 573–581, 1966.
Mothes, K.: The role of kinetin in plant regulation.—5th intern. Conf. Plant Growth Substances. Pp. 131–140. Gif.-Sur-Yvette 1963.
Munzel, E.: Hemmung der Regeneration anBegonia rex Blattstücken durch Gibberellin.—Naturwissenschaften55: 659, 1968.
Paulet, P.: Étude de la néoformationin vitro de bourgeons végétatifs et floraux.—Thèse Dr. Sci. 105 p. (Librairie Générale de l'Enseignement).—Paris 1968.
Prevot, P.: Influence de l'hétéroauxine (acide 3-indolacétique) sur la néoformation des bourgeons chezBegonia rex Putz.—Bull. Soc. roy. Sci. (Liège)3–4: 284–288, 1938.
Prevot, P.: Contribution à l'histologie des phénomènes de néoformation chezBegonia rex Putz.—Rev. sci.5: 275–285, 1948.
Schraudolf, H., Reinert, J.: Interaction of plant growth regulators in regeneration processes. —Nature184: 465–466, 1959.
Skoog, F., Miller, C. O.: Chemical regulation of growth and organ formation in plant tissues culturedin vitro.—Symp. Soc. exp. Biol.11: 118–131, 1957.
Tran Thanh Van, M.: Organisation des bourgeons à partir de l'épiderme des fragments de feuilles deBegonia rex Putz. Localisation des synthèses des acides nucléiques au cours de cette néoformation.—Rev. gén. Bot.77: 125–136, 1970.
Wirth, K.: Experimentelle Beeinflussung der Organbildung anin vitro kultivierten Blattstücken vonBegonia rex.—Planta54: 265–293, 1960.
Zachau, H. G., Dutting, D., Feldmann, H.: Nucleotide sequences of two serine-specific transfer ribonucleic acids.—Angew. Chem. Intern. Edit.5: 422, 1966.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chlyah, A. Néoformation caulinaire et radiculaire chez les fragments de feuille deBegonia rex Putz.: Action de divers facteurs régulateurs trophiques et d'environnement. Biol Plant 14, 204–212 (1972). https://doi.org/10.1007/BF02921251
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02921251