Biologia Plantarum

, 35:131 | Cite as

PSTV infection in tobacco (Nicotiana tabacum L.) transformed with PSTV cDNA

  • J. Matoušek
  • S. Rakouský
  • L. Trněná
  • D. Riesner
Brief Communication


Tobacco cv. White Burley was transformed with disarmed expression vector pCB1314 containing dimeric cDNA of potato spindle tuber viroid (PSTV, severe strain) in plus orientation regulated from the mannopine promoter. Amount of PSTV specific (−) and (+) sequences and PSTV circular forms was measured in transformed tobacco stock and compared with PSTV content in untransformed tomato and tobacco grafts. It follows from the results that lower rate of accumulation of PSTV in tobacco as compared with tomato is due to less intensive viroid transportation through the cytoplasm and/or cell to cell moverment, whereas both, viroid replication and processing showed comparable characteristics in tomato and tobacco with respect to accumulation of minus and plus strands and circular forms in infected tissues. Despite of accumulation of viroid in comparable amount in both transformed tobacco and infected tomato, no expression of any morphological symptom of disease was observed in transgenic tobacco.


Transgenic Tobacco Potato Spindle Tuber Viroid Infected Tomato Infected Tobacco Viroid Infection 
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Copyright information

© Institute of Experimental Botany 1993

Authors and Affiliations

  • J. Matoušek
    • 1
  • S. Rakouský
    • 1
  • L. Trněná
    • 1
  • D. Riesner
    • 2
  1. 1.Institute of Plant Molecular BiologyCzechoslovak Academy of SciencesČeské BudějoviceCzech Republic
  2. 2.Institute of Physical BiologyDüsseldorf UniversityDüsseldorfGermany

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