Biologia Plantarum

, Volume 36, Issue 2, pp 277–284 | Cite as

Proline metabolic pathways in calli fromLycopersicon esculentum andL. pennellii under salt stress

  • A. Rus-Alvarez
  • G. Guerrier
Original Papers


The enzyme activities of the proline metabolic pathways were determined in control and satt-treated (140 M NaCl) calli derived from cotyledons of the domestic saltsensitive tomatoLycopersicon esculentum and the wild salt-tolerantL. pennellii. Glutamate, glutamine, asparagine, and aspartate levels increased in both genotypes under salt stress, while proline accumulation increased markedly only in the salt sensitive tomato. Activity of glutamine synthetase (GS) decreased in the salt-treated calli of the domestic species, whereas both NADH- and NADPH-glutamate synthase (GOGAT) activities increased; GS and NADPH-GOGAT decreased together in the salinized calli of the wild species. Decreasing ornithine levels were found due to NaCl in both tomato populations, while ornithine transaminase (OT) decreased in the wild type only. Increasing NADPH-Δ-pyrroline-5-carboxylate reductase (P5CR) and decreasing proline oxidase (Pro oxi) occurred in the salinized calli of the wild type. Conversely, Pro oxi and proline dehydrogenase (Pro dH) decreased highly in the salinized calli of the domestic population, while no significant changes in P5CR were found.


Proline Salt Stress Glutamine Synthetase Relative Growth Rate Proline Accumulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



dichlorophenol 2,6 indophenol




flavin adenine dinucleotide


glutamate synthase


glutamine synthetase


ornithine transaminase


Δ-pyrroline-5-carboxylate reductase

Pro dH

proline dehydrogenase

Pro oxi

proline oxidase


relative growth rate


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Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • A. Rus-Alvarez
    • 1
  • G. Guerrier
    • 1
  1. 1.Groupe de Biochimie et de Biologie Moléculaire Végétales, Ensemble ScientifiqueAngers CedexFrance

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