Increase of callus and embryoid production from hypocotyl protoplasts of sunflower (Helianthus annuus L.) by culture in microdrops
Hypocotyls of seedling plants of the sunflower inbred line HNK-81 were used as a source of protoplasts. Optimal conditions of isolation and culture of protoplasts were established. Using the method of individual culture in microdrops, a 77% final plating efficiency was achieved. Cytokinins, especially zeatin, showed a significant influence on the formation of globular embryo-like structures, but these failed to develop into mature embryos. Calli obtained in liquid media containing auxins and cytokinins grew on agar solidified media without growth regulators.
KeywordsCellulase Zeatin Cell Coloni Mature Embryo Protoplast Culture
- Dupuis, J.M., Gomiero, M., Plantevin, C., Chagvardieff, P.: Différenciacion de formes embryoides issues de protoplastes de tournesol (Helianthus annuus L.).—Compt. rend. Acad. Sci. Paris307: 465–468, 1988.Google Scholar
- Fischer, Ch., Hahne, G.: Sunflower cotyledon protoplasts. Their development in liquid and solidified medium.—Physiol. Plant.82(Suppl.): A112, 1991.Google Scholar
- Krasnyanski, S., Menczel, L.: Shoot regeneration from protoplast derived callus of sunflower (Helianthus annuus L.).—Physiol. Plant.82 (Suppl.): A14, 1991.Google Scholar
- Moyne, A.L., Thor, V., Pélissier, B., Bergounioux, C., Freyssinet, G., Gadal, P.: Callus and embryoid formation from protoplasts ofHelianthus annuus.—Plant Cell Rep.7: 437–440, 1988.Google Scholar
- Schmitz, P., Shnabl, B.: Regeneration and evacuolation of protoplasts from mesophyll, hypocotyl and petioles fromHelianthus annuus L..—J. Plant Physiol.135: 223–227, 1989.Google Scholar