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Biologia Plantarum

, 36:183 | Cite as

Increase of callus and embryoid production from hypocotyl protoplasts of sunflower (Helianthus annuus L.) by culture in microdrops

  • J. Šamaj
  • A. Okolot
  • M. Bobak
  • Yu. Gleba
Original Papers

Abstract

Hypocotyls of seedling plants of the sunflower inbred line HNK-81 were used as a source of protoplasts. Optimal conditions of isolation and culture of protoplasts were established. Using the method of individual culture in microdrops, a 77% final plating efficiency was achieved. Cytokinins, especially zeatin, showed a significant influence on the formation of globular embryo-like structures, but these failed to develop into mature embryos. Calli obtained in liquid media containing auxins and cytokinins grew on agar solidified media without growth regulators.

Keywords

Cellulase Zeatin Cell Coloni Mature Embryo Protoplast Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BAP

6-benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

KIN

kinetin

MS

Murashige-Skoog medium

NAA

1-naphthaleneacetic acid

ZEA

zeatin

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Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • J. Šamaj
    • 1
  • A. Okolot
    • 2
  • M. Bobak
    • 1
  • Yu. Gleba
    • 2
  1. 1.Department of Plant PhysiologyComenius UniversityBratislavaSlovak Republic
  2. 2.Institute of Cell Biology and EngineeringKiev GSP 22Ukraine

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