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Purification, electron microscopy and serology of virus isolated fromEuonymus europaea

Purilfikácia, elektrónová mikroskopia a sérologia vírusu izolovaného z bršlena európskeho

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Biologia Plantarum

Abstract

“Euonymus mosaic virus”, purified from cucumber cotyledons by the differential and density-gradient centrifugation, shows typical nucleoprotein absorption spectrum. Electron microscopy reveals isometric virus particles of about 37 nm diameter. No reaction of purified “Euonymus mosaic virus” was observed with antisera against a raspberry ringspot virus, tobacco ringspot virus, cherry leaf roll virus, strawberry latent ringspot virus, tomato ringspot virus, elm mosaic virus, arabis mosaic virus, tomato bushy stunt virus and watermelon mosaic virus.

Abstract

„Virus mozaiky bršlena” purifikovaný z klíčných listov uhoriek diferenciálnou a hustotnou gradientovou centrifugáciou vykazuje typické absorpčné spektrum nukleoproteinu. Pod elektronovým mikroskopom je možné vidiet izometrické vírusové častice s priemerom okolo 37 nm. Vyčistený „vírus mozaiky bršlena” nereagoval s antisérom proti vírusu krúžkovitosti maliny, vírusu krúžkovitosti rajčiaka, vírusu mozaiky brestu, vírusu mozaiky arábky, vírusu zakrpatenosti rajčiaka a vírusu mozaiky dyne červenej.

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Authors and Affiliations

  1. Institute of Experimental Phytopathology and Entomology, Slovak Academy of Sciences, Ivánka pri Dunaji, Czechoslovakia

    Valéria Šubíková, Helena Baumgartnerová & V. Bojňanský

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  1. Valéria Šubíková
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  2. Helena Baumgartnerová
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  3. V. Bojňanský
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Šubíková, V., Baumgartnerová, H. & Bojňanský, V. Purification, electron microscopy and serology of virus isolated fromEuonymus europaea. Biol Plant 16, 321–324 (1974). https://doi.org/10.1007/BF02920991

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  • DOI: https://doi.org/10.1007/BF02920991

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