Biologia Plantarum

, 36:397 | Cite as

Biointeraction between precarthamin and cell components in florets ofCarthamus tinctorius L.

  • K. Saito
Original Papers


The relative rate of precarthamin extractability and floret protein inactivation by organic solvents were compared. Upon trituration of the floral tissues ofCarthamus tinctorius L., usually less extractable precarthamin is released at a markedly high level, while the releasability rate is changed conspicuously by macerating the florets in the test solvents of increased concentrations; the amount of releasable precarthamin also varies according to the solvents used. 30% (v/v) acetone promotes the pigment solubility about twice, whereas, its capacity decreases abruptly in reverse proportion to the increment of acetone content. Methanol accelerates the precarthamin release, but the rate is very low. Ethanol acts in far lesser extent. The data indicate that these varied aspects are more or less attributed to the inactivation of possible factors dissociating precarthamin from cellular components in freezed safflower florets.


Flavonoid Floral Tissue Flower Petal Test Solvent Carthamus Tinctorius 
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  1. Kreuzaler, F., Hahlbrock, K.: Flavonoid glycosides from illuminated cell suspension cultures ofPetroselinum hortense.—Phytochemistry12: 1149–1152, 1973.CrossRefGoogle Scholar
  2. McClure, J.W.: Physiology and function of flavonoids.—In: Harborne, J.B., Mabry, T.J., Mabry, H. (ed.): The Flavonoids. Pp. 970–1055. Academic Press, New York 1975.Google Scholar
  3. Saito, K.: Possible site of flavonoid synthesis in the photosynthetic apparatus.—Biochem. J.144: 431–432, 1974.PubMedGoogle Scholar
  4. Saito, K., Katsukura, M.: The chemical interaction between precarthamin and cellular components.— Biochem. Physiol. Pflanzen188: 399–404, 1993.Google Scholar
  5. Saito, K., Yamamoto, T.: The enzymatic mobilization of bound precarthamine from the flower florets ofCarthamus tinctorius L.—Biol. Plant.36: 403–407, 1994.CrossRefGoogle Scholar
  6. Sasse, F., Backs-Husemann, D., Barz, W.: Isolation and characterization of vacuoles from cell suspension cultures ofDaucus carota.—Z. Naturforsch.34c: 848–854, 1979.Google Scholar
  7. Sanders, J.A., McClure, J.W., Wallace, J.W.: The subcellular localization of enzymes in the flavonoid biosynthetic pathway.—Amer. J. Bot.60 (Suppl.): 29, 1973.Google Scholar
  8. Schulz, M., Weissenböck, G.: Isolation and separation of epidermal and mesophyll protoplasts from rye primary leaves—tissue-specific characteristics of secondary phenolic product accumulation.— Z. Naturforsch.41c: 22–27, 1986.Google Scholar
  9. Takahashi, Y., Miyasaka, T., Tasaka, S., Urano, S., Ikura, M., Hikichi, K., Matsumoto, T., Wada, M.: Constitution of two coloring matters in the flower petals ofCarthamus tinctorius L.— Tetrahedron Lett.23: 5163–5166, 1982.CrossRefGoogle Scholar
  10. Takahashi, Y., Saito, K., Yanagiya, M., Ikura, M., Hikichi, K., Matsumoto, T., Wada, M.: Chemical constitution of safflor yellow B, a quinochalcone C-glycoside from flower petals ofCarthamus tinctorius L.—Tetrahedron Lett.25: 2471–2474, 1984.CrossRefGoogle Scholar
  11. Weissenböck, G.: Experiments on the localization of flavonoids in plastids. II. Comparison of the flavonoids of isolated etioplasts and chloroplasts ofAvena sativa.—Ber. deut. bot. Ges.86: 351–359, 1973.Google Scholar

Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • K. Saito
    • 1
  1. 1.Department of Bioscience and Technology, School of EngineeringHokkaido Tokai UniversitySapporoJapan

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