Biologia Plantarum

, 36:343 | Cite as

Somatic embryogenesis inCicer arietinum L: Influence of genotype and auxins

  • S. Eapen
  • L. George
Original Papers


Plant regeneration through somatic embryogenesis was obtained in chickpea (Cicer arietinum L.) using immature cotyledons and immature embryonal axes as explants. 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3.5-6-trichloropicolinic acid (picloram) and 3.6-dichloro-0-anisc, acid (dicamba) in concentrations 1, 2, 5, 10 mg dm−3 were found better than naphthaleneacetic acid (NAA) (10–20 mg dm−3) for the induction of globular and heart-shaped somatic embryos. The embryos developed upto the dicotyledonary stage on medium supplemented with saccharose, mannitol and silver nitrate (AgNO3) and developed further into plantlets on medium containing gibberellic acid (GA3) and abscisic acid (ABA). The frequency of somatic embryogenesis was dependent on the genotype and auxins used.


Somatic Embryo Somatic Embryogenesis Plant Regeneration Embryonal Axis Picloram 
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Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • S. Eapen
    • 1
  • L. George
    • 1
  1. 1.Plant Biotechnology SectionBhabha Atomic Research CentreBombayIndia

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