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Biologia Plantarum

, Volume 11, Issue 6, pp 432–441 | Cite as

Cytogenetic studies on the effect of chronic gamma irradiation onVicia faba

  • Soheir M. Amer
  • I. Y. Mostafa
  • Evon Mikhael
Article
  • 33 Downloads

Abstract

Vicia faba seeds (cv. Giza 1) were planted in the Inshas gamma radiation field where they were chronically irradiated during the whole life of the plant. The percentage of the induced abnormal P.M.Cs, as well as the frequency of abnormal P.M.Cs in the different meiotic stages were proportional with the given doses. The main types of chromosome aberrations were anaphase and telophase bridges, fragmentation and lagging chromosomes. The nearest plants to the source showed an inhibition of shoot growth, flower and seed sterility and irregular branching. At the dosage levels used irradiation had no effect on pollen fertility.

Seeds of the 1st filial generation were used for both mitotic and meiotic studies. The percentage of the mitotic abnormalities was proportional with the doses. The most dominant type of anomaly was the presence of micronuclei in the different stages of mitosis and in the resting cells. Irradiation affected also other types of anomaliese.g. lagging chromosomes, fragments, bridges...etc.

Meiosis, and pollen fertility (2nd generation) were normal.

Keywords

Pollen Fertility Pollen Mother Cell Chronic Irradiation Meiotic Stage Seed Sterility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Cytogenetické studium vlivu chronického gama záření naVicia faba

Abstract

SemenaVicia faba byla vyseta na gama pole a rostliny byly chronicky ozařovány po celou vegetační dobu. Procento indukovaných abnormálních PMC a frekvence abnormálních PMC v různých meiotických stadiích byly přímo úměrné použité dosi. Nejčastějšími typy chromosomálních aberací byly můstky v anafázi a telofázi, fragmenty, lagging chromosomy. Rostliny, které byly nejblíže zdroji záření vykazovaly inhibici odnožování, sterilitu květů a semen a nepravidelné větvení. Použité dose záření neovlivňovaly fertilitu pylu. Semena prvé filiární generace byla použita pro studium mitosy a rostliny F1 pro studium meiose. Procento meiotických abnormalit bylo přímo úměrné dosi. Nejčastějším typem anomálií byla přítomnost mikrojader v různých stadiích mitosy i v interfázi. Záření indukovalo i další typy anomálií, jako lagging chromosomy, fragmenty, můstky atd. Meiose i fertility pylu v generaci, následující po ozařování, byly normální.

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Copyright information

© Institute of Experimental Botany 1969

Authors and Affiliations

  • Soheir M. Amer
    • 1
    • 2
  • I. Y. Mostafa
    • 1
    • 2
  • Evon Mikhael
    • 1
    • 2
  1. 1.National Research CentreCairo
  2. 2.Radiobiology DepartmentAtomic Energy EstablishmentCairoU.A.R.

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