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Accumulation of biodegradable copolyesters of 3-hydroxy-butyrate and 3-hydroxyvalerate inAlcaligenes eutrophus

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Abstract

Biodegradable copolyesters of 3-hydroxybutyrate-co-3-hydroxyvalerate (3HB-3HV) were produced byAlcaligenes eutrophus in a two-staged process, namely growth stage and nitrogen-deficient polyester-accumulation stage. When C5 was used as the sole carbon source, the copolyester contained 43 mol % of 3HV. A range of copolyesters with 0-43 mol % of 3HV could be produced by using a medium containing different concentration ratios of butyric acid C4 and C5. Tm of PHB homopolymer was 177.6©C and that of copolyester with highest 3HV mol fraction of 43% was 99.0©C. C5 concentration in the medium could be an effective means to control the polymeric composition and mechanical properties of the copolyesters accumulated in A.eutrophus.

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References

  1. Chua, H., Yu, P. H. F., Xing, S., and Ho, L. Y. (1995),J. Plast. Technol. 21, 65–73.

    Google Scholar 

  2. Hong Kong Environmental Protection Department (1994), Environment Hong Kong 1994, Hong Kong Government Press, pp. 51 – 66.

  3. Hong Kong and Kowloon Plastic Product Merchants United Association (1992), Hong KongPlast. Ind. Bull., 33, December Issue.

  4. Hong Kong Government Industry Department (1993), Hong Kong’s Manufact. Industry 1993, Hong Kong Government Press.

  5. Billmeyer, F. W. (1971),Polymer Science, Wiley, New York, pp. 379–490.

    Google Scholar 

  6. Huang, T., Zhao, J. Q., and Shen, J. R. (1991),Plast. Ind.,4, 23–27.

    Google Scholar 

  7. Young, R. J. (1981),Introduction to Polymers, Chapman and Hall, New York pp. 9–85.

    Google Scholar 

  8. Emballage Digest (1988),Emballage Dig. 30, 80.

    Google Scholar 

  9. Chua, H., Yu, P. H. F. and Hu, W. F. (1996),Proceedings of the 12th International Conference on Solid Waste Technology Management Nov. 17 – 20,1996, University of Pennsylvania.

  10. Ho, L. Y., (1997), Master Thesis, The Hong Kong Polytechnic University.

  11. Industrie-Anzeiger (1987),Industrie-Anzeiger,109, 26.

    Google Scholar 

  12. Pelissero, A. (1987),Imballaggio,38, 54.

    Google Scholar 

  13. Pfeffer, J. T. (1992),Solid Waste Manage, Eng. 72 – 84.

  14. Shen J. R., Zhao, J. Q., Huang, T., and Chen, S. M. (1994),Better Living Through Innovative Biochemical Engineering, (Teo, W. K. et al, ed., Singapore University Press, Singapore 843–845.

    Google Scholar 

  15. Linko, S., Vaheri, H., and Seppala, J. (1993),Appl. Microbiol. Biotechnol. 39, 11–15.

    CAS  Google Scholar 

  16. Yamane, T. (1993),Biotechnol. Bioeng. 41, 165–170.

    Article  CAS  Google Scholar 

  17. Kumagai, Y. (1992),Polym. Degrad. Stabil. 37, 253–256.

    Article  CAS  Google Scholar 

  18. Lee, S. Y., Chang, H. N., and Chang, Y. K. (1994),Better Living Through Innovative Biochem. Eng., Teo, W. K., ed., Singapore University Press, pp. 53 – 55.

  19. Shirai, Y., Yamaguchi, M., Kusubayashi, N., Hibi, K., Uemura, T., and Hashimoto, K. (1994),Better Living Through Innovative Biochem. Eng., Teo, W. K., ed., Singapore University Press, pp. 263 – 265.

  20. Shimizu, H., Sonoo, S., Shioya, S., and Suga, K. (1992),Biochem. Eng. for 2001, Furusaki et al. ed., Springer-Verlag, Tokyo, pp. 195–197.

    Google Scholar 

  21. Chua, H., Yu, P. H. F., and Ho, L. Y. (1997),Appl. Biochem. Biotechnol. 63, 627–635.

    Article  Google Scholar 

  22. Chua, H., Hu, W. F., and Ho, L. Y. (1997),J. IES,37(2), 9–13.

    Google Scholar 

  23. Doi, Y. (1990). Microb. Polyesters. VCH. New York.

    Google Scholar 

  24. Bluhm, T. L., Hamer, G. K., Marchessault, R. H., Fyfe, C. A., and Veregin, R. P. (1986),Macromolecules 19, 2871–2876.

    Article  CAS  Google Scholar 

  25. Bloemnergen, S., Holden, D. A., Harmer, G. K., Bluhm, T. L., and Marchessault, R. H. (1986),Macromolecules 19, 2865–2871.

    Article  Google Scholar 

  26. Cox, M. K. (1994),Biodegrad. Plast. Polym., Doi et al., ed., Elsevier, Tokyo, pp. 120–135.

    Google Scholar 

  27. Doi, Y., Kanesawa, Y., Kunioka, M., and Saito, T. (1990),Macromolecules 23, 26–31.

    Article  CAS  Google Scholar 

  28. Mergaert, J., Webb, A., Anderson, C, Wouters, A., and Swings, J. (1993),Appl. Environ. Microbiol.,59, 3233–3238.

    CAS  Google Scholar 

  29. Kim, G. J., Yun, K. Y., Bae, K. S., and Rhee, Y. H. (1992),Biotechnol. Lett. 14(1), 27–32.

    Article  CAS  Google Scholar 

  30. APHA (1995),Standard Methods for Examination of Waste and Wastewater, 19th ed., APHA, AWWA, WPCF, Washington, DC.

    Google Scholar 

  31. Ishihara, Y., Shimizu, H., and Shioya, S. (1996),J. Ferment. Bioeng. 81, 422–428.

    Article  CAS  Google Scholar 

  32. Doi, Y., Tamaki, A., Kunioka, M., and Soga, K. (1988),Appl. Microbiol. Biotecnol. 28, 330–334.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Departments of Civil and Structural Engineering, The Hong Kong Polytechnic University, Hung Horn, Hong Kong

    H. Chua

  2. Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Horn, Hong Kong

    P. H. F. Yu & W. Lo

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  1. H. Chua
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  2. P. H. F. Yu
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  3. W. Lo
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Chua, H., Yu, P.H.F. & Lo, W. Accumulation of biodegradable copolyesters of 3-hydroxy-butyrate and 3-hydroxyvalerate inAlcaligenes eutrophus . Appl Biochem Biotechnol 70, 929–935 (1998). https://doi.org/10.1007/BF02920203

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