Applied Biochemistry and Biotechnology

, Volume 70, Issue 1, pp 579–592 | Cite as

Phenomenological and neural-network modeling of cephalosporin C production bioprocess

  • A. J. G. Cruz
  • M. L. G. C. Araujo
  • R. C. Giordano
  • C. O. Hokka
Session 3: Bioprocessing Research


Cephalosporin C production process withCephalosporium acremonium ATCC 48272 in synthetic medium was investigated and the experimental results allowed the development of a mathematical model describing the process behavior. The model was able to explain fairly well the diauxic phenomenon, higher growth rate during the glucose-consumption phase, and the production occurring only in the sucrose-consumption phase.

Moreover, the process was simulated utilizing the neural-networks technique. Two feed-forward neural-networks with one hidden layer were employed. Both models, phenomenological and neural-networks based, satisfactorily describe the bioprocess. The difficulties in determining kinetic parameters are avoided when neural networks are utilized.

Index Entries

Cephalosporium acremonium cephalosporin C phenomenological modeling neural network diauxic phenomenon 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    El-Sayed, A.-H. M. M. (1992), inHandbook of Applied Mycology, vol. 4, Arora, D. K., ed., Banaras Hindu University, Varanasi, India, pp. 517–564.Google Scholar
  2. 2.
    Behmer, C. J. and Demain, A. L. (1983),Current Microbiol. 8, 107–114.CrossRefGoogle Scholar
  3. 3.
    Matsumura, M., Imanaka, T., Yoshida, T., and Taguchi, H. (1981),J. Ferment. Technol. 59^(2), 115–123.Google Scholar
  4. 4.
    Chu, W. B. Z and Constantinides, A. (1988),Biotechnol. Bioeng. 32, 277–288.CrossRefGoogle Scholar
  5. 5.
    Rumelhart, D. E. and McClelland, J. L. (1986), inParallel Distributed Processing: explorations in the microstructure of cognition, vol. 1, MIT, Cambridge, MA, pp. 318–362.Google Scholar
  6. 6.
    Thibault, J., Breusegem, Van V., and Chéruy, A. (1990),Biotechnol. Bioeng. 36, 1041–1048.CrossRefGoogle Scholar
  7. 7.
    DiMassimo, C., Montague, G. A., Willis, M. J., Tham, M. T., and Morris, A. J. (1992),Computers Chem. Eng. 16^(4), 283–291.CrossRefGoogle Scholar
  8. 8.
    Syu, M.-J and Tsao, G. T. (1993),Biotechnol. Bioeng. 42, 376–380.CrossRefGoogle Scholar
  9. 9.
    Cruz, A. J. G., Hokka, C. O., and Giordano, R. C. (1996),Proceedings of the 11st Simpósio Nacional de Fermentações (XI SINAFERM, São Carlos, SP, Brasil.) 1, pp. 84–89.Google Scholar
  10. 10.
    Cruz, A. J. G, Hokka, C. O., and Giordano, R. C. (1996),Proceedings of the 3rd Congreso Interamericano de Computacion Aplicada a la Industria de Procesos (CAIP ’96, Villa Maria, Cordoba, Republica da Argentina), pp. 115–118.Google Scholar
  11. 11.
    Shen, Y.-Q, Wolfe, S., and Demain, A. L. (1986),Bioeng. Biotechnol. 4, 61–63.Google Scholar
  12. 12.
    Demain, A. L., Newkirk, J. F., and Hendlin, D. (1963),J. Bacterial. 85, 339–344.Google Scholar
  13. 13.
    Barham, D., and Trinder, P. (1972), Analyst,97(2), 142–145.CrossRefGoogle Scholar
  14. 14.
    Claridge, C. A., and Johnson, D. L. (1962),Antimicrobiol. Ag. Chemother. 682–686.Google Scholar
  15. 15.
    Araujo, M. L. G. C., Oliveira, R. P., Giordano, R. C., and Hokka, C. O. (1996),Chem. Eng. Sci. 51(11), 2835–2840.CrossRefGoogle Scholar
  16. 16.
    Petzold, L. (1989), Subroutine DDASSL, Computing and Mathematics Research Division, Lawrence Livermore National Laboratory, Livermore, CA.Google Scholar
  17. 17.
    Marquardt, D. W. (1963),J. Soc. Indust. Appl. Math. 11, 431–441.CrossRefGoogle Scholar
  18. 18.
    Bhat, N. and McAvoy, T. J. (1990),Computers Chem. Eng. 14(4/5), 573–583.CrossRefGoogle Scholar
  19. 19.
    Bailey, J. E. and Ollis, D. F. (1986),Biochemical Engineering Fundamentals, 2nd ed., McGraw-Hill, New York.Google Scholar
  20. 20.
    Bajpai, R. K., and Reuss, M. (1981),Biotechnol. Bioeng. 22, 739–763.Google Scholar
  21. 21.
    Araujo, M. L. G. C. (1996),PhD Thesis, Chemical Engineering Department, Federal University of São Carlos, SP, Brazil.Google Scholar
  22. 22.
    Cruz, A. J. G. and Giordano, R. C. (1996),Proceedings of the 11st Brazilian Congress of Chemical Engineering (11 COBEQ, Rio de Janeiro, RJ), 571–576.Google Scholar

Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • A. J. G. Cruz
    • 1
  • M. L. G. C. Araujo
    • 1
  • R. C. Giordano
    • 1
  • C. O. Hokka
    • 1
  1. 1.Departamento de Engenharia QuímicaUniversidade Federal de São CarlosSão Carlos, SPBrazil

Personalised recommendations