Abstract
Cephalosporin C production process withCephalosporium acremonium ATCC 48272 in synthetic medium was investigated and the experimental results allowed the development of a mathematical model describing the process behavior. The model was able to explain fairly well the diauxic phenomenon, higher growth rate during the glucose-consumption phase, and the production occurring only in the sucrose-consumption phase.
Moreover, the process was simulated utilizing the neural-networks technique. Two feed-forward neural-networks with one hidden layer were employed. Both models, phenomenological and neural-networks based, satisfactorily describe the bioprocess. The difficulties in determining kinetic parameters are avoided when neural networks are utilized.
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References
El-Sayed, A.-H. M. M. (1992), inHandbook of Applied Mycology, vol. 4, Arora, D. K., ed., Banaras Hindu University, Varanasi, India, pp. 517–564.
Behmer, C. J. and Demain, A. L. (1983),Current Microbiol. 8, 107–114.
Matsumura, M., Imanaka, T., Yoshida, T., and Taguchi, H. (1981),J. Ferment. Technol. 59^(2), 115–123.
Chu, W. B. Z and Constantinides, A. (1988),Biotechnol. Bioeng. 32, 277–288.
Rumelhart, D. E. and McClelland, J. L. (1986), inParallel Distributed Processing: explorations in the microstructure of cognition, vol. 1, MIT, Cambridge, MA, pp. 318–362.
Thibault, J., Breusegem, Van V., and Chéruy, A. (1990),Biotechnol. Bioeng. 36, 1041–1048.
DiMassimo, C., Montague, G. A., Willis, M. J., Tham, M. T., and Morris, A. J. (1992),Computers Chem. Eng. 16^(4), 283–291.
Syu, M.-J and Tsao, G. T. (1993),Biotechnol. Bioeng. 42, 376–380.
Cruz, A. J. G., Hokka, C. O., and Giordano, R. C. (1996),Proceedings of the 11st Simpósio Nacional de Fermentações (XI SINAFERM, São Carlos, SP, Brasil.) 1, pp. 84–89.
Cruz, A. J. G, Hokka, C. O., and Giordano, R. C. (1996),Proceedings of the 3rd Congreso Interamericano de Computacion Aplicada a la Industria de Procesos (CAIP ’96, Villa Maria, Cordoba, Republica da Argentina), pp. 115–118.
Shen, Y.-Q, Wolfe, S., and Demain, A. L. (1986),Bioeng. Biotechnol. 4, 61–63.
Demain, A. L., Newkirk, J. F., and Hendlin, D. (1963),J. Bacterial. 85, 339–344.
Barham, D., and Trinder, P. (1972), Analyst,97(2), 142–145.
Claridge, C. A., and Johnson, D. L. (1962),Antimicrobiol. Ag. Chemother. 682–686.
Araujo, M. L. G. C., Oliveira, R. P., Giordano, R. C., and Hokka, C. O. (1996),Chem. Eng. Sci. 51(11), 2835–2840.
Petzold, L. (1989), Subroutine DDASSL, Computing and Mathematics Research Division, Lawrence Livermore National Laboratory, Livermore, CA.
Marquardt, D. W. (1963),J. Soc. Indust. Appl. Math. 11, 431–441.
Bhat, N. and McAvoy, T. J. (1990),Computers Chem. Eng. 14(4/5), 573–583.
Bailey, J. E. and Ollis, D. F. (1986),Biochemical Engineering Fundamentals, 2nd ed., McGraw-Hill, New York.
Bajpai, R. K., and Reuss, M. (1981),Biotechnol. Bioeng. 22, 739–763.
Araujo, M. L. G. C. (1996),PhD Thesis, Chemical Engineering Department, Federal University of São Carlos, SP, Brazil.
Cruz, A. J. G. and Giordano, R. C. (1996),Proceedings of the 11st Brazilian Congress of Chemical Engineering (11 COBEQ, Rio de Janeiro, RJ), 571–576.
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Cruz, A.J.G., Araujo, M.L.G.C., Giordano, R.C. et al. Phenomenological and neural-network modeling of cephalosporin C production bioprocess. Appl Biochem Biotechnol 70, 579–592 (1998). https://doi.org/10.1007/BF02920170
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DOI: https://doi.org/10.1007/BF02920170