Applied Biochemistry and Biotechnology

, Volume 70, Issue 1, pp 441–462 | Cite as

Ethanol production from AFEX-treated forages and agricultural residues

  • Khaled Belkacemi
  • Ginette Turcotte
  • Damien de Halleux
  • Philippe Savoie
Session 3: Bioprocessing Research


Lignocellulosic materials derived from forages, namely timothy grass, alfalfa, reed canary grass, and agricultural residues, such as corn stalks and barley straw, were pretreated using ammonia fiber explosion (AFEX) process. The pretreated materials were directly saccharified by cellulolytic enzymes. Sixty to 80% of theoretical yield of sugars were obtained from the pretreated biomasses. Subsequent ethanolic fermentation of the hydrolysates byPachysolen tannophilus ATCC 32691 resulted in 40-60% of theoretical yield after 24 h, based on the sugars present in the hydrolysates. The uptake of sugars was not complete, indicating a possible inhibitory effect onP. tannophilus during the fermentation of these substrates.

Index Entries

Forages agricultural residues AFEX enzymatic hydrolysis ethanolic fermentation biofuel 


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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Khaled Belkacemi
    • 1
  • Ginette Turcotte
    • 1
  • Damien de Halleux
    • 2
  • Philippe Savoie
    • 2
    • 3
  1. 1.Department of Food Science and NutritionAgri-Food Engineering University LavalQuébecCanada
  2. 2.Department of SoilAgri-Food Engineering University LavalQuébecCanada
  3. 3.Agriculture and Agri-Food CanadaSte-FoyCanada

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