Pretreatment of sugarcane bagasse hemicellulose hydrolysate for xylitol production byCandida guilliermondii
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In order to remove or reduce the concentrations of toxic substances present in the sugarcane bagasse hemicellulose hydrolysate for xyloseto-xylitol bioconversion, the hydrolysate was pretreated by changing the initial pH level through the combination of different bases and acids with or without the subsequent addition of activated charcoal. Attention was given to the influence of the fermentation time as well.
The experiments were based on multivariate statistical concepts, with the application of fractional factorial design techniques to identify the important variables in the process. Subsequently, the levels of these variables were quantified by the response surface methodology, which permitted the establishment of the best pretreatment procedure with a xylose-to-xylitol bioconversion efficiency of 86.2%. This procedure consisted in increasing the pH of the hydrolysate from 0.5 to 7.0 with CaO and reducing it to 5.5 with H3PO4. Next, the hydrolysate was treated with activated charcoal (2.4%). The highest xylitol yield (0.79 g/g) corresponded to a productivity of 0.47 g/L/h.
Index EntriesSugarcane bagasse hydrolysate pretreatment xylitol Candida guilliermondii
- 3.Melaja, A. J. and Hämäläinen, L. (1997),Process for Making Xylitol. US Patent no. 4.008.285. 1975, 15 fev.Google Scholar
- 11.Box, G. E. P., Hunter, W. G., and Hunter, J. S. (1978),Statistics for Experimenters: An Introduction to Design, Data Analysis and Model Building. Wiley, New York.Google Scholar
- 12.Barros Neto, B., Scarminio, I. S., and Bruns, R. E. (1995),Planejamento e Otimizaççao de Experimentos, 1 ed., Editora da UNICAMP, Campinas.Google Scholar
- 14.Felipe, M. G. A., Mancilha, I. M, Vitolo, M., Roberto, I. C, Silva, S. S., and Rosa, S. A. M. (1993),Arauivos de Biologia e Technologia,36, 103–114.Google Scholar