Advertisement

Journal of Ocean University of China

, Volume 5, Issue 2, pp 119–122 | Cite as

Construction of a metagenomic DNA library of sponge symbionts and screening of antibacterial metabolites

  • Chen Juan
  • Zhu Tianjiao
  • Li Dehai
  • Cui Chengbin
  • Fang Yuchun
  • Liu Hongbing
  • Liu Peipei
  • Gu Qianqun
  • Zhu Weiming
Research Papers
  • 139 Downloads

Abstract

To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of spongeGelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper dise assaying. Two clones displayed the antibacterial activity againstMicrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the hostE. coli DH5α and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts.

Key words

metagenomic DNA library sponge symbionts metabolite bioactivity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bewley, C. A. and D. J. Faulkner, 1998. Lithistid sponges: star performers or hosts to the stars.Angewandte Chemie International Edition,37: 2162–2178.CrossRefGoogle Scholar
  2. Brady, S. F., and J. Clardy, 2000. Long-chain N-Acyl amino acid antibiotics isolated from heterologously expressed environmental DNA.J. Am. Chem. Soc.,122: 12903–12904.CrossRefGoogle Scholar
  3. Brady, S. F., C. J. Chao, J. Handelsman, and J. Clardy, 2001. Cloning and heterologous expression of a natural product biosynthetic gene cluster from eDNA.Org. Lett.,3: 1981–1984.CrossRefGoogle Scholar
  4. Brady, S. F., C. J. Chao, and J. Clardy, 2002. New natural product families from an environmental DNA (eDNA) gene cluster.J. Am. Chem. Soc.,124: 9968–9969.CrossRefGoogle Scholar
  5. Brady, S. F., and J. Clardy, 2004. Palmitoylputrescine, an antibiotic isolated from the heterologous expression of DNA extracted from bromeliad tank water.J. Nat. Prod.,67: 1283–1286.CrossRefGoogle Scholar
  6. Hildebrand, M., L. E. Waggoner, G. E. Lim, K. H. Sharp, C. P. Ridley,et al., 2004. Approaches to identify, clone, and express symbiont bioactive metabolite genes.Nat. Prod. Rep.,21: 122–142.CrossRefGoogle Scholar
  7. Mearns-Spragg, A., M. Bregu, K. G. Boyd, and J. G. Burgess, 1998. Cross-species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria.Lett. Appl. Microbiol.,27: 142–146.CrossRefGoogle Scholar
  8. Unson, M. D., and D. J. Faulkner, 1993. Cyanobacterial symbiont biosynthesis of chlorinated metabolites fromDysidea herbacea.Experientia,49: 349–353.CrossRefGoogle Scholar
  9. Unson, M. D., N. D. Holland, and D. J. Faulkner, 1994. A brominated secondary metabolite synthesized by the cyanobacterial symbiont of marine sponge and accumulation of the crystalline metabolite in the sponge tissue.Mar. Biol.,119: 1–11.CrossRefGoogle Scholar
  10. Wang, G. Y. S., E. Graziani, B. Waters, W. B. Pars, X. Li,et al., 2000. Novel natural products from soil DNA libraries in a streptomycete host.Org. Lett.,2: 2401–2404.CrossRefGoogle Scholar

Copyright information

© Journal of Ocean University of China and Science Press 2006

Authors and Affiliations

  • Chen Juan
    • 1
  • Zhu Tianjiao
    • 1
  • Li Dehai
    • 1
  • Cui Chengbin
    • 2
  • Fang Yuchun
    • 1
  • Liu Hongbing
    • 1
  • Liu Peipei
    • 1
  • Gu Qianqun
    • 1
  • Zhu Weiming
    • 1
  1. 1.Key Laboratory of Marine Drugs of Chinese Ministry of Education, Institute of Marine Drug and FoodOcean University of ChinaQingdaoP.R. China
  2. 2.Beijing Institute of Pharmacology and ToxicologyAMMSBeijingP.R. China

Personalised recommendations