Skip to main content
SpringerLink
Log in

Granulocyte activation

Biochemical events associated with the mobilization of calcium

  • Symposium
  • Published:
Survey of Immunologic Research Aims and scope Submit manuscript

Summary

The past few years have seen a significant refinement in our understanding of the mechanism of activation of granulocytes. The involvement of surface receptors and the role of calcium as second messenger have been further supported by recent data. However, with the exception of the unexamined potential role of ITP, all the previous hypotheses attempting to provide a biochemical basis for the transduction mechanisms involved in calcium mobilization have either been dismissed or at the least been seriously weakened by lack of experimental support. A particularly glaring example of the work that still needs to be done is the near total lack of knowledge about stimuli-induced changes in the physical properties of the plasma membrane of these cells and of the biochemical targets of the raised levels of cytoplasmic calcium (e.g. protein kinases). Glimpses into the nature of the calcium-independent pathway of neutrophil activation have been provided by phorbol esters. The modulatory nature of the role of cyclic mucleotides appears to have been established, its biochemical basis, however, is unknown. The description of the dynamic nature of the neutrophils’ cytoskeletal structures should lay the foundation of the understanding of the regulation of the neutrophils’ force generating system, the structure(s) ultimately responsible for the expression of the critical granulocyte functions of locomotion, phagocytosis, and granule fusion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bareis, D.L.; Hirata, F.; Schiffmann, E.; Axelrod, J.: Phospholipid metabolism, calcium flux, and the receptor mediated induction of chemotaxis in rabbit neutrophils. J. Cell Biol.93: 690–697 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Bormann, B.J.; Huang, C.-K.; Mackin, W.M.; Becker, E.L.: Receptor-mediated activation of a phospholipase A2 in rabbit neutrophil plasma membrane. Proc. natn. Acad. Sci. USA (in press, 1984).

  • Chandler, D.; Meusel, G.; Schumaker, E.; Stapleton, D.: FMLP-induced enzyme release from neutrophils: a role for intracellular calcium. Am. J. Physiol.245: C196-C202 (1983).

    PubMed  CAS  Google Scholar 

  • Chenoweth, D.E.; Goodman, M.G.: The C5a receptor of neutrophils and macrophages; in Keller, Till, Leukocyte locomotion and chemotaxis, pp. 252–269 (Birkhäuser, Basel 1983).

    Google Scholar 

  • Coates, T.D.; Torres, M.; Williams, L.V.; Sisken, J.E.; Baehner, R.L.: Localization of the chlorotetracycline labelled pool of trigger calcium in human neutrophils by quantitative video intensification microscopy. J. Cell Biol.97: 420a (1983).

    Google Scholar 

  • Cockcroft, S.: Phosphatidylinositol metabolism in mast cells and neutrophils. Cell Calcium3: 337–349 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Downs, P.; Michell, R.H.: Phosphatidylinositol 4-phosphate and phosphatidylinositol 4, 5-bisphosphate: lipids in search of a function. Cell Calcium3: 467–502 (1982).

    Article  Google Scholar 

  • Fletcher, M.P.; Gallin, J.I.: Human neutrophils contain an intracellular pool of putative receptors for the chemoattractant N-formyl-methionyl-leucyl-phenylalanine. Blood62: 792–799 (1983).

    PubMed  CAS  Google Scholar 

  • Garcia-Castro, I.; Mato, J.M.; Vasanthakuman, G.; Wiesmann, W.P.; Schiffmann, E.; Chiang, P.K.: Paradoxical effects of adenosine on neutrophil chemotaxis. J. biol. Chem.255: 4345–4549 (1983).

    Google Scholar 

  • Goldman, D.W.; Goetzl, E.J.: Specific binding of leukotriene B4 to receptors on human polymorphonuclear leukocytes. J. Immun.129: 1600–1604 (1982).

    PubMed  CAS  Google Scholar 

  • Gomperts, B.: Involvement of guanine nucleotide binding protein in the gating of Ca2+ by receptors. Nature, Lond.306: 64–66 (1983).

    Article  CAS  Google Scholar 

  • Holmes, R.P.; Yoss, N.L.: Failure of phosphatidic acid to translocate Ca2+ across phosphatidylcholine membranes. Nature, Lond.305: 637–638 (1983).

    Article  CAS  Google Scholar 

  • Huang, C.-K.; Hill, J.M.; Bormann, B.-J.; Mackin, W.M.; Becker, E.L.: Endogenous substrates for cyclic AMP-dependent and calcium-dependent protein phosphorylation in rabbit peritoneal neutrophils. Biochim. biophys. Acta.760: 126–135 (1983).

    PubMed  CAS  Google Scholar 

  • Jackowski, S.; Sha’afi, R.I.: Response of adenosine cyclic 3′, 5′-monophosphate level in rabbit neutrophils to the chemotactic peptide formyl-methionyl-leucyl-phenylalanine. Molec. Pharmacol.16: 473–481 (1979).

    CAS  Google Scholar 

  • Jesaitis, A.J.; Naemura, J.R.; Painter, R.G.; Sklar, L.A.; Cochrane, C.G.: The fate of an N-formylated chemotactic peptide in stimulated human granulocytes. J. biol. Chem.258: 1969–1977 (1983).

    Google Scholar 

  • Koo, C.; Lefkowitz, R.I.; Snyderman, R.: The oligopeptide chemotactic factor receptor on human polymorphonuclear leukocyte membranes exists in two affinity states. Biochem. biophys. Res. Commun.106: 442–449 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Mackin, W.M.; Huang, C.-K.; Becker, E.L.: The formyl peptide chemotactic receptor on rabbit peritoneal neutrophils. J. Immun.129: 1608–1611 (1982).

    PubMed  CAS  Google Scholar 

  • Michell, R.H.: Inositol phospholipids and cell surface receptor function. Biochim. biophys. Acta415: 81–147 (1975).

    PubMed  CAS  Google Scholar 

  • Naccache, P.H.; Sha’afi, R.I.: Arachidonic acid, leukotriene B4 and neutrophil activation. Ann. N.Y. Acad. Sci. (in press, 1984).

  • Niedel, J.E.; Cuatrecasas, P.: Formyl peptide chemotactic receptors of leukocytes and macrophages. Curr. Top. cell. Regul.17: 137–170 (1980).

    PubMed  CAS  Google Scholar 

  • Palmer, R.J.J.; Salmon, J.A.: Release of leukotriene B4 from human neutrophils and its relationship to degranulation induced by N-formyl-methionyl-leucyl-phenylalanine, serum treated zymosan and the ionophore A23187. Immunology.50: 65–73 (1983).

    PubMed  CAS  Google Scholar 

  • Pozzan, T.; Lew, D.P.; Wollheim, C.B.; Tsien, R.Y.: Is cytosolic ionized calcium regulating neutrophil activation? Science221: 1413–1415 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Schiffmann, E.; Corcoran, B.A.; Wahl, S.A.: N-for-mylmethionyl peptides as chemoattractants for leucocytes. Proc. natn. Acad. Sci. USA72: 1059–1062 (1975).

    Article  CAS  Google Scholar 

  • Schmitt, M.; Painter, R.C.; Jesaitis, A.J.; Preissner, K.; Sklar, L.A.; Cochrane, C.G.: Photoaffinity labelling of the N-formyl peptide receptor binding site of intact human polymorphonuclear leukocytes. J. biol. Chem.258: 649–654 (1983).

    PubMed  CAS  Google Scholar 

  • Schneider, C; Zanetti, M.; Romeo, D.: Surface reactive stimuli selectively increase protein phosphorylation in human neutrophils. FEBS Lett.127: 4–8 (1981).

    Article  PubMed  CAS  Google Scholar 

  • Serhan, C; Anderson, P.; Goodman, E.; Dunham, P.; Weissmann, G.: Phosphatidate and oxidized fatty acids are calcium ?onophores J. biol. Chem.256: 2736–2741 (1982).

    Google Scholar 

  • Sha’afi, R.I.; Naccache, P.H.: Ionic events in neutrophil chemotaxis and secretion. Adv. Inflammation Res., vol. 2, pp. 115–148 (Raven Press, New York 1981).

    Google Scholar 

  • Sha’afi, R.I.; White, J.R.; Molski, T.F.P.; Shefcyk, J.; Volpi, M.; Naccache, P.H.; Feinstein, M.B.: Phorbol 12-myristate 13-acetate activates rabbit neutrophils without an apparent rise in the level of intracellular calcium. Biochem. biophys. Res. Commun.114: 638–645 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Simchowitz, L.; Spilberg, I.; Atkinson, J.P.: Evidence that the functional responses of human neutrophils occur independently of transient elevations in cAMP levels. J. cyclic Nucleotide Prot. Phosphoryl. Res.9: 35–47 (1983).

    CAS  Google Scholar 

  • Sirois, P.; Borgeat, P.: Mediators of immediate hypersensitivity. Immunopharmacology4: 201–222 (1982).

    Article  Google Scholar 

  • Snyderman, R.; Edge, S.; Pike, M.C.: Macrophage chemotactic factor receptor exists in two interconvertible affinities modulated by guanine nucleotides. Clin. Res.30: 521A (1982).

    Google Scholar 

  • Snyderman, R.; Goetzl, E.J.: Molecular and cellular mechanisms of leukocyte chemotaxis. Science213: 830–837 (1981).

    Article  PubMed  CAS  Google Scholar 

  • Streb, H.; Irvine, R.F.; Berridge, M.J.; Shulz, I.: Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate. Nature, Lond.306: 67–69 (1983).

    Article  CAS  Google Scholar 

  • Tsien, R.Y.: A non-disruptive technique for loading calcium buffers and indicators into cells. Nature Lond.290: 527–528 (1981).

    Article  PubMed  CAS  Google Scholar 

  • Volpi, M.; Yassin, R.; Naccache, P.H.; Sha’afi, R.I.: Chemotactic factor causes rapid decrease in phosphatidylinositol 4, 5-bis-phosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophils. Biochem. biophys. Res. Commun.112: 951–964 (1983).

    Article  Google Scholar 

  • White, J.R.; Huang, C.-K.; Hill, J.; Naccache, P.H.; Becker, E.L.; Sha’afi, R.I.: Effect of PMA and its analogue 4-α-PDD on protein phosphorylation, actin association with cytoskeleton, lysozomal enzyme release and cell aggregation in rabbit neutrophils (submitted for publication 1984).

  • White, J.R.; Naccache, P.H.; Molski, T.F.P.; Borgeat, P.; Sha’afi, R.I.: Direct demonstration of increased intracellular concentration of free calcium in rabbit and human neutrophils following stimulation by chemotactic factor. Biochem. biophys. Res. Commun.113: 44–50 (1983a).

    Article  PubMed  CAS  Google Scholar 

  • White, J.R.; Naccache, P.H.; Sha’afi, R.I.: Stimulation by chemotactic factor of actin association with the cytoskeleton in rabbit neutrophils. Effects of calcium and cytochalasin B. J. biol. Chem.258: 14041–14047 (1983b).

    CAS  Google Scholar 

  • Young, J.D.-E.; Unkeless, J.C.; Young, T.M.; Mauro, A.; Cohn, Z.A.: Role for mouse macrophage IgG Fc receptor as ligand-dependent ion channel. Nature, Lond.306: 186–189 (1983).

    Article  CAS  Google Scholar 

  • Zigmond, S.H.; Sullivan, S.T.; Lauffenburger, D.A.: Kinetic analysis of chemotactic peptide receptor modulation. J. Cell Biol.92: 34–43 (1983).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Pathology and Physiology, University of Connecticut Health Center, 06032, Farmington, CT, USA

    P. H. Naccache & R. I. Sha’afi

Authors
  1. P. H. Naccache
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. I. Sha’afi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported in part by NIH grants AM-31000, AI-09648, and AI-13734.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Naccache, P.H., Sha’afi, R.I. Granulocyte activation. Surv. immunol. Res. 3, 288–294 (1984). https://doi.org/10.1007/BF02919046

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02919046

Keywords

Search

Navigation