Abstract
Two alternative enzymatic routes to continuous biological acetaldehyde production from ethanol by immobilized yeast were compared: ADH ofCandida utilis and AOX ofCandida boidinii. The ADH route, performed by immobilized cells in the presence of tris buffer continuously trapping the acetaldehyde formed, was found superior to the AOX route. It exhibited significantly higher operational stability in both batchwise or continuous operation in fluidized bed reactor. The ADH system exhibited a stable acetaldehyde production level for 5 d, followed by gradual decrease in activity, which could be readily fully regeneratedin situ by flushing with growth medium. Efficient continuous product trapping by tris buffer was essential to maintain continuous operation.
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Shachar-Nishri, Y., Freeman, A. Continuous production of acetaldehyde by immobilized yeast within situ product trapping. Appl Biochem Biotechnol 39, 387–399 (1993). https://doi.org/10.1007/BF02919005
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DOI: https://doi.org/10.1007/BF02919005