Applied Biochemistry and Biotechnology

, Volume 39, Issue 1, pp 323–335 | Cite as

Strain screening and development for industrial lactic acid fermentation

  • S. P. Tsai
  • R. D. Coleman
  • S. H. Moon
  • K. A. Schneider
  • C. Sanville Millard
Session 3 Applied Biological Research II


Thirty-four lactic acid bacterial strains have been screened for their potential in industrial lactic acid fermentations. The screening criteria included substrate utilization, product yield, resistance to product inhibition, product stereospecificity, growth temperature, fermentation rate, and ease of cell-broth separation. Acclimation techniques were also used to increase growth temperature and improve product tolerance. This effort has resulted in the identification and improvement of several promising strians, and a superior mixed culture. The mixed culture exhibited a >98% product stereospecificity, cell viability at 106.6 g/L lactic acid, and a productivity of 5.3 g/(L·h) in batch fermentation.

Index Entries

Lactic acid fermentation strain development stereospecific product inhibition 


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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • S. P. Tsai
    • 1
  • R. D. Coleman
    • 1
  • S. H. Moon
    • 1
  • K. A. Schneider
    • 1
  • C. Sanville Millard
    • 1
  1. 1.Argonne National LaboratoryArgonne

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