Abstract
Thirty-four lactic acid bacterial strains have been screened for their potential in industrial lactic acid fermentations. The screening criteria included substrate utilization, product yield, resistance to product inhibition, product stereospecificity, growth temperature, fermentation rate, and ease of cell-broth separation. Acclimation techniques were also used to increase growth temperature and improve product tolerance. This effort has resulted in the identification and improvement of several promising strians, and a superior mixed culture. The mixed culture exhibited a >98% product stereospecificity, cell viability at 106.6 g/L lactic acid, and a productivity of 5.3 g/(L·h) in batch fermentation.
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The submitted manuscript has been authored by a contractor of the US Government under contract No. W-31-109-ENG-38. Accordingly, the US Government retains a nonexclusive, royalty free license to publish or reproduce the published form of this contribution, or allow others to do so, for US Government purposes.
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Tsai, S.P., Coleman, R.D., Moon, S.H. et al. Strain screening and development for industrial lactic acid fermentation. Appl Biochem Biotechnol 39, 323–335 (1993). https://doi.org/10.1007/BF02919000
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DOI: https://doi.org/10.1007/BF02919000