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Studies on protein methyltransferase in human cerebrospinal fluid

  • Jongok Park
  • Jeffrey I. Greenstein
  • Woon Ki Paik
  • Sangduk Kim
Article

Abstract

Protein methyltransferases, rich in most mammalian brains, were studied in human cerebrospinal fluid (CSF). Among several well-characterized groups of methyltransferases, protein methylase I (S-adenosyl-methionine:protein-arginineN-methyltransferase, EC 2.1.1.23) was found in significant amounts in human CSF samples. Both myelin basic protein (MBP)-specific and histone-specific protein methylase I activities were observed, the latter being generally higher in most CSF.S-Adenosyl-l-homocysteine, a potent product inhibitor for the methyltransferase, inhibited approximately 90% of MBP-specific protein methylase I activity at a concentration of 1 mM. The optimum pH of the MBP-specific protein methylase I was found to be around 7.2. Identity of exogenously added MBP as the methylated substrate for CSF enzyme was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. An amino acid analysis of the [methyl-3H]protein hydrolysate showed two major radioactive peaks cochromatographing with monomethyl- and dimethyl (symmetric)-arginine. Human CSF contained relatively high endogenous protein methylase I activity (activity measured without added substrate protein): The endogenous substrate can be immunoprecipitated by antibody raised against calf brain MBP. Finally, CSF from several neurological patients were analyzed for protein methylase I, and the results are presented.

Keywords

Myelin Basic Protein Basic Amino Acid AdoMet Methionine Adenosyltransferase Automatic Amino Acid Analyzer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1989

Authors and Affiliations

  • Jongok Park
    • 1
  • Jeffrey I. Greenstein
    • 2
  • Woon Ki Paik
    • 1
  • Sangduk Kim
    • 1
  1. 1.Fels Research InstituteTemple University School of MedicinePhiladelphiaUSA
  2. 2.Department of NeurologyTemple University School of MedicinePhiladelphiaUSA

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