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Ricerca in clinica e in laboratorio

, Volume 18, Issue 2–3, pp 121–142 | Cite as

Nucleic acid hybridization in viral Hepatitis research

  • Ferruccio Bonino
  • Elisabetta Chiaberge
  • Maurizia Rossana Brunetto
  • Paolo Piantino
  • Eleonora Maran
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Summary

Nucleic acid hybridization is extremely important in viral hepatitis research and the newly emerging techniques are now becoming an indispensable resource also in the diagnostic laboratory. This paper provides information on methods currendy used for detection of viral nucleic acids with special emphasis on the importance of hepatitis B virus DNA in the serum. Herein, we describe the procedures for preparation and labeling of DNA probes and the principles that regulate dot, slot and Southern blot hybridization. Advantages and shortcomings of filter hybridization are discussed together with the ahernadve approaches to it. Finally, a collection of laboratory protocols is presented inAppendix "#1.

Key-words

DNA probe assays Hepatitis B virus DNA Hepatitis viruses Hybridization Nucleic acids 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Ferruccio Bonino
    • 1
  • Elisabetta Chiaberge
    • 1
  • Maurizia Rossana Brunetto
    • 1
  • Paolo Piantino
    • 1
  • Eleonora Maran
    • 1
  1. 1.Laboratorio di Epatologia, Divisione di GastroenterologiaOspedale MolinetteTorinoItalia

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