Abstract
Water-soluble polymers immobilized by gamma radiation have been investigated as a means of developing electrochemical sensors. Enzyme-based sensors for glucose and lactate have been made by immobilizing glucose oxidase and lactate oxidase, respectively, on platinized graphite electrodes. The enzyme is entrapped in a polymeric network of poly(vinyl alcohol) that is formed by gamma radiation crosslinking. Electrodes coated with poly (N-vinylpyrrolidone) and its corresponding monomer and then crosslinked with gamma radiation show an extraction of catecholamines into the polymer film that enhances the analytical signal for their detection by electrochemical oxidation. Poly(dimethyldiallylammonium chloride) spin-coated on a screen-printed electrochemical cell provides sufficient ionic conductivity for the cell to function as a gas sensor for oxygen, which is detected by reduction at a platinum working electrode.
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De Castro, E. S., Huber, E. W., Villarroel, D., Galiatsatos, C., Mark, J. E., Heineman, W. R., and Murray, P. T. (1987),Anal. Chem. 59, 134–139.
Huber, E. W. and Heineman, W. R. (1988),Anal. Chem. 60, 2467–2472.
Coury, Jr., L. A., Birch, E. M., and Heineman, W. R. (1988),Anal. Chem. 60, 553–560.
Coury, Jr., L. A., Huber, E. W., Birch, E. M., and Heineman, W. R. (1989),J. Electrochem. Soc. 136, 1044–1049.
Galiatsatos, C., Ikariyama, Y., Mark, J. E., and Heineman, W. R. (1990),Biosens. & Bioelect. 5, 47–61.
Birch, M. E., Coury, Jr., L. A. and Heineman, W. R. (1990),Anal. Chem. 62, 1123–1130.
Hajizadeh, K. (1990), Ph. D. Dissertation, University of Cincinnati.
Hajizadeh, K., Halsall, H. B., and Heineman, W. R. (1991),Anal. Chim. Acta 243, 23–32.
Turner, A. P. F., Karube, I., and Wilson G. S. (1987),Biosensors: Fundamentals and Applications, Oxford University Press, New York.
Imato, T., Morioka, H., and Heineman, W. R. (1992), 4th International Meeting on Chemical Sensors, Tokyo, Japan, September 13–18.
Tieman, R. S., Heineman, W. R., Johnson, J., and Seguin, R. (1992),Sens. & Actuators B 8, 199–204.
Tieman, R. S., Igo, D. H., Heineman, W. R., Johnson, J., and Seguin, R. (1991),Sens. & Actuators B 5, 121–127.
Tieman, R. S. and Heineman, W. R. (1992),Anal. Lett. 25, 807–819.
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Heineman, W.R. Biosensors based on polymer networks formed by gamma irradiation crosslinking. Appl Biochem Biotechnol 41, 87–97 (1993). https://doi.org/10.1007/BF02918535
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DOI: https://doi.org/10.1007/BF02918535