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Immunologic Research

, Volume 13, Issue 1, pp 42–48 | Cite as

Production of multiple murine CD2 receptor constructs using the baculovirus expression vector and a rapid dot-blot assay

  • Adam J. Kaplan
  • Kenneth D. Chavin
  • Li Hui Qin
  • Hideo Yagita
  • Jonathan S. Bromberg
Article
  • 26 Downloads

Abstract

The baculovirus expression system was used to produce three different constructs of the murine cell surface adhesion receptor CD2. One construct coded for a single, N-terminal, Ig-fold domain. It was inefficiently secreted and therefore primarily intracellular. The second construct coded for both extracellular, N-terminal Ig-fold domains. This was efficiently secreted into culture supernatant. The third construct coded for the full-length transmembrane molecule which localized to the cell surface. All constructs were monomers of predicted MWr and were appropriately glycosylated. They retained epitopic specificity as demonstrated by binding to mAbs, and adhesion function as demonstrated by a rosetting assay.

Key Words

CD2 CD48 Baculovirus Adhesion 

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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Adam J. Kaplan
    • 1
    • 2
  • Kenneth D. Chavin
    • 1
    • 2
  • Li Hui Qin
    • 2
  • Hideo Yagita
    • 3
  • Jonathan S. Bromberg
    • 1
    • 2
    • 4
  1. 1.Department of SurgeryMedica University of South CarolinaCharlestonUSA
  2. 2.Microbiology and ImmunologyMedical University of South CarolinaCharlestonUSA
  3. 3.Department of ImmunologyJuntendo University, School of MedicineTokyoJapan
  4. 4.Department of SurgeryUniversity of MichiganAnn ArborUSA

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